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Specification of the Hindbrain Fate in the Zebrafish

Woo, Katherine and Fraser, Scott E. (1998) Specification of the Hindbrain Fate in the Zebrafish. Developmental Biology, 197 (2). pp. 283-296. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160322-114552568

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Abstract

We determine the timing of neural commitment by hindbrain tissue in the zebrafish using microsurgical transplantation. When transplanted at shield stage to the ventral side of the embryo, presumptive hindbrain cells are not committed, as they can adapt to their environment and give rise to epidermis. In contrast, when transplanted at 80% epiboly, hindbrain cells retain their neural fate and express neural-specific antigens. Moreover, they are able to maintain regional fate, as is evident by the expression of the hindbrain-specific marker, Krox20. In addition, we observe that committed hindbrain tissues are able to induce presumptive ventral epidermis to form neural crest derivatives, otic vesicles, and neural tissues. We propose that hindbrain progenitors have acquired regional identity as a group at 80% epiboly even before making vertical contact with axial mesoderm. These results suggest that planar induction may constitute a significant component in the zebrafish neural patterning pathway.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/dbio.1998.8870DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160698988704PublisherArticle
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 1998 Academic Press. Available online 9 April 2002. We thank T. Jowett, S. Shulte-Merker (via B. Trevarrow), B. Trevarrow, and E. Weinberg for reagents. K.W. thanks her thesis committee members at Caltech and J. Shih for experimental suggestions and encouragement. Thanks also to A. Grove, C. Krull, M. Selleck, and J. Shih for critical reading of the manuscript, and two anonymous reviewers for constructive comments. Finally, we dedicate this paper to the memory of Dr. J. Oppenheimer, who had inspired us to pursue transplantation studies in the zebrafish. Work was supported by the NIH (MH49176).
Funders:
Funding AgencyGrant Number
NIHMH49176
Subject Keywords: zebrafish; Danio rerio; neural patterning; hindbrain; otic placode; neural crest; microsurgery
Issue or Number:2
Record Number:CaltechAUTHORS:20160322-114552568
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160322-114552568
Official Citation:Katherine Woo, Scott E Fraser, Specification of the Hindbrain Fate in the Zebrafish, Developmental Biology, Volume 197, Issue 2, 15 May 1998, Pages 283-296, ISSN 0012-1606, http://dx.doi.org/10.1006/dbio.1998.8870. (http://www.sciencedirect.com/science/article/pii/S0012160698988704)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65593
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:22 Mar 2016 20:43
Last Modified:03 Oct 2019 09:48

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