A Caltech Library Service

In Vivo Regulation of Somite Differentiation and Proliferation by Sonic Hedgehog

Marcelle, Christophe and Ahlgren, Sara and Bronner-Fraser, Marianne (1999) In Vivo Regulation of Somite Differentiation and Proliferation by Sonic Hedgehog. Developmental Biology, 214 (2). pp. 277-287. ISSN 0012-1606. doi:10.1006/dbio.1999.9389.

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item:


In vertebrates, somite differentiation is mediated in part by Sonic Hedgehog (Shh), secreted by the notochord and the floor plate. However, Shh-null mice display close to normal expression of molecular markers for dermomytome, myotome, and sclerotome, indicating that Shh might not be required for their initial induction. In this paper, we have addressed the capacity of Shh to regulate in vivo the expression of the somite differentiation markers Pax-1, MyoD, and Pax-3 after separation of paraxial mesoderm from axial structures. We show that Pax-1, which is lost under these experimental conditions, is rescued by Shh. In contrast, Shh maintains, but cannot induce MyoD expression, while Pax-3 expression is independent of the presence of axial structures or Shh. Finally, we demonstrate that Shh is a potent mitogen for somitic cells, supporting the idea that it may serve to expand subpopulations of cells within the somite.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1999 Academic Press. Received for publication March 16, 1999. Revised June 16, 1999. Accepted June 16, 1999. Available online 2 April 2002. We are grateful to the following for their generous gifts of reagents used in this study: Dr. Rudy Balling for the Pax-1 probe, Dr. Cliff Tabin for the Shh probe, and Dr. Bruce Patterson for the chick MyoD probe. This work was supported by grants from the Muscular Dystrophy Foundation to C.M. and M.B.F., NIH Grant NSO 7251 to S.A., and NIH Grant 34671 to M.B.F.
Funding AgencyGrant Number
Muscular Dystrophy FoundationUNSPECIFIED
Subject Keywords:Shh; somite; proliferation; BrdU; Pax-1; MyoD; Pax-3
Issue or Number:2
Record Number:CaltechAUTHORS:20160323-071054297
Persistent URL:
Official Citation:Christophe Marcelle, Sara Ahlgren, Marianne Bronner-Fraser, In Vivo Regulation of Somite Differentiation and Proliferation by Sonic Hedgehog, Developmental Biology, Volume 214, Issue 2, 15 October 1999, Pages 277-287, ISSN 0012-1606, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65603
Deposited By: Ruth Sustaita
Deposited On:23 Mar 2016 15:22
Last Modified:10 Nov 2021 23:47

Repository Staff Only: item control page