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Leukemia Inhibitory Factor Is Expressed in Astrocytes Following Cortical Brain Injury

Banner, Lisa R. and Moayeri, N. Nicole and Patterson, Paul H. (1997) Leukemia Inhibitory Factor Is Expressed in Astrocytes Following Cortical Brain Injury. Experimental Neurology, 147 (1). pp. 1-9. ISSN 0014-4886. doi:10.1006/exnr.1997.6536.

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The neuropoietic cytokine leukemia inhibitory factor (LIF) can act as a trophic factor, enhancing neuronal survival, and as a differentiation factor altering neuronal and glial gene expression. LIF also plays a role in the response to injury of the peripheral nervous system, as indicated by an increase in the amount of its mRNA within nonneuronal cells following nerve damage and lack of neuronal injury response in LIF knockout mice. To determine if LIF is regulated after injury to the central nervous system, we surgically lesioned the cortex in adult rat brain. Using a quantitative RNAse protection assay, we find that LIF mRNA increases 30-fold following injury. The amount of this transcript goes up within 6 h after injury, reaches a peak at 24 h and returns to baseline by 7 days postlesion.In situhybridization analysis reveals LIF transcript-containing cells scattered throughout the ipsilateral cortex close, but not immediately adjacent to the lesion site. Double-labeling with a variety of antibodies reveals that LIF mRNA is induced in GFAP-positive astrocytes as well as in a small number of microglial cells. The striking induction of LIF transcripts in glia suggests that this cytokine may play a key injury–response role in the CNS as it does in the PNS, where LIF has been demonstrated to regulate neuropeptide expression bothin vivoandin vitro.

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Additional Information:© 1997 by Academic Press. Available online 18 April 2002. We thank Herman Govan and Reto Gadient for constructive comments on the manuscript. This project was supported by National Research Service Awards (L.R.B. and N.N.M.) and the National Institute of Neurological Disorders and Stroke (P.H.P.).
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National Research Service AwardUNSPECIFIED
National Institute of Neurological Disorders and Stroke (NINDS)UNSPECIFIED
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Official Citation:Lisa R. Banner, N. Nicole Moayeri, Paul H. Patterson, Leukemia Inhibitory Factor Is Expressed in Astrocytes Following Cortical Brain Injury, Experimental Neurology, Volume 147, Issue 1, September 1997, Pages 1-9, ISSN 0014-4886, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65609
Deposited By: Ruth Sustaita
Deposited On:23 Mar 2016 17:01
Last Modified:10 Nov 2021 23:47

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