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Spatial expression of a forkhead homologue in the sea urchin embryo

Harada, Yoshito and Akasaka, Koji and Shimada, Hiraku and Peterson, Kevin J. and Davidson, Eric H. and Satoh, Noriyuki (1996) Spatial expression of a forkhead homologue in the sea urchin embryo. Mechanisms of Development, 60 (2). pp. 163-173. ISSN 0925-4773. doi:10.1016/S0925-4773(96)00608-9.

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Echinoderms are the sister group of the chordates and hemichordates within the deuterostomes. They lack a notochord or any structures obviously homologous with it. To gain insight into developmental mechanisms important in the origin and early evolution of chordates, we investigated sea urchin homologues of chordate genes that are implicated in notochord formation, viz. Brachyury and HNF-3β. Here we report the pattern of expression of a sea urchin orthologue of forkhead, Hphnf3 which is present as a single copy per haploid genome. An Hphnf3 transcript of 3.0 kb was first detected at the swimming blastula stage, accumulated maximally at the gastrula and prism-embryo stages, and decreased at the pluteus-larva stage. In situ hybridization signals were found in cells of the vetetal plate of the swimming blastula. During gastrulation, intense staining was evident in the cells surrounding the blastopore, whereas weak staining was detected in the invaginating archenteron. At the prism-embryo stage, the entire archenteron stained intensely; then, at pluteus stage, the larva staining decreased in intensity. The forkhead and Brachyury genes begin to be expressed almost simultaneously in sea urchin embryos, in the vegetal plate at the late blastula stage. After the onset of gastrulation, however, Hphnf3 is expressed in the posterior part of the archenteron, whereas the Brachyury orthologue, HpTa, is expressed in the secondary mesenchyme founder cells, which occupy the anterior tip of archenteron. Hphnf3 may contribute to specification of embryonic cells as archenteron, and the role of HpTa may be directed towards specification of mesodermal founder cells. Except for the basal character of expression in endoderm and endomesoderm, these transcription factors are clearly utilized differently in chordates.

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Additional Information:© 1996 Elsevier Science Ireland Ltd. Received 22 July 1996; revision received 12 September 1996; accepted 17 September 1996. Available online 23 March 1999. Y.H. was supported by a Predoctoral Fellowship from the Japan Society for the Promotion of Science for Japanese Junior Scientists with Research Grant #6575. This research was supported by Grants-in-Aid for Specially Promoted Research (No. 07102012) and for International Scientific Research (No. 06044120) from the Ministry of Education, Science, Sports and Culture of Japan to N.S. and Grants-in-Aid from the same Ministry (Nos. 07458195 and 07262211) to H.S. The nucleotide sequence data reported in this paper will appear in the DDBJ, EMBL and GenBank Nucleotide Sequence Databases with the accession number (D83182).
Funding AgencyGrant Number
Japan Society for the Promotion of Science (JSPS)6575
Grants-in-Aid for Specially Promoted Research07102012
Ministry of Education, Science, Sports and Culture of Japan06044120
Ministry of Education, Science, Sports and Culture of Japan07458195
Ministry of Education, Science, Sports and Culture of Japan07262211
Subject Keywords:Sea urchin embryo; Forkhead/HNF-3 gene; Sequence conservation; Vegetal plate cells; Archenteron; Brachyury gene
Issue or Number:2
Record Number:CaltechAUTHORS:20160323-135305463
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Official Citation:Yoshito Harada, Koji Akasaka, Hiraku Shimada, Kevin J. Peterson, Eric H. Davidson, Noriyuki Satoh, Spatial expression of a forkhead homologue in the sea urchin embryo, Mechanisms of Development, Volume 60, Issue 2, December 1996, Pages 163-173, ISSN 0925-4773, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65629
Deposited On:24 Mar 2016 17:40
Last Modified:10 Nov 2021 23:48

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