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Age-Dependent Neurotransmitter Plasticity of Ciliary Ganglion Neurons

Sechrist, John W. and Wolf, John and Bronner-Fraser, Marianne (1998) Age-Dependent Neurotransmitter Plasticity of Ciliary Ganglion Neurons. Molecular and Cellular Neuroscience, 12 (4-5). pp. 311-323. ISSN 1044-7431. doi:10.1006/mcne.1998.0720.

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We have examined neurotransmitter plasticity in postmitotic cholinergic neurons isolated from 6.5- to 11-day-old embryonic quail ciliary ganglia. Purified neurons were labeled with DiI, transplanted into the trunk of young chick embryos, and assayed for catecholamine content and [^3H]thymidine uptake 4 to 5 days later. For ciliary neurons derived from 6.5- to 8-day-old embryos, as many as 25% (average of 9% overall) expressed catecholamines in the host sympathetic ganglia, migratory stream, aortic plexuses, and adrenal medulla. In contrast, neurons from >8-day-old ganglia did not acquire or produce detectable catecholamines, indicating a limited time period over which phenotypic conversion can occurin vivo.As a control, ciliary neurons were also injected into the head mesenchyme of young embryos; no catecholamine expression was observed. Interestingly, after transplantation some Dil-labeled postmitotic ciliary neurons took up [^3H]thymidine with or without phenotypic change. These results suggest that phenotypic plasticity in ciliary neurons is age-dependent, is location-dependent, and may involve resumption of DNA replication, a characteristic feature of some differentiating adrenergic sympathetic neurons. Apoptosis of a few proliferating transplanted cells may be induced independently or in association with transmitter change.

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Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1998 Academic Press. Received August 20, 1998. Revised September 25, 1998. Accepted September 28, 1998. Available online 25 May 2002. We thank Dr. J. Coulombe for advice and preliminary assistance with cell purification procedures and Dr. Sara Ahlgren for input on apoptosis. This work was supported by NS 36585 and NS34671.
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NIHNS 36585
Issue or Number:4-5
Record Number:CaltechAUTHORS:20160324-065910513
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Official Citation:John W. Sechrist, John Wolf, Marianne Bronner-Fraser, Age-Dependent Neurotransmitter Plasticity of Ciliary Ganglion Neurons, Molecular and Cellular Neuroscience, Volume 12, Issues 4–5, November 1998, Pages 311-323, ISSN 1044-7431, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65638
Deposited By: Ruth Sustaita
Deposited On:24 Mar 2016 16:10
Last Modified:10 Nov 2021 23:48

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