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Restriction Fragment Length Polymorphism Linkage Map for Arabidopsis thaliana

Chang, Caren and Bowman, John L. and DeJohn, Arthur W. and Lander, Eric S. and Meyerowitz, Elliot M. (1988) Restriction Fragment Length Polymorphism Linkage Map for Arabidopsis thaliana. Proceedings of the National Academy of Sciences of the United States of America, 85 (18). pp. 6856-6860. ISSN 0027-8424. PMCID PMC282077.

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We have constructed a restriction fragment length polymorphism linkage map for the nuclear genome of the flowering plant Arabidopsis thaliana. The map, containing 90 randomly distributed molecular markers, is physically very dense; >50% of the genome is within 1.9 centimorgans, or approx 270 kilobase pairs, of the mapped DNA fragments. The map was based on the meiotic segregation of markers in two different crosses. The restriction fragment length polymorphism linkage groups were integrated with the five classically mapped linkage groups by virtue of mapped mutations included in these crosses. Markers consist of both cloned Arabidopsis genes and random low-copy-number genomic DNA clones that are able to detect polymorphisms with the restriction enzymes EcoRI, Bgl II, and/or Xba I. These cloned markers can serve as starting points for chromosome walking, allowing for the isolation of Arabidopsis genes of known map location. The restriction fragment length polymorphism map also can associate clones of unknown gene function with mutant phenotypes, and vice versa.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Lander, Eric S.0000-0003-2662-4631
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1988 National Academy of Sciences. Communicated by E. B. Lewis, June 8, 1988. We are grateful to the following people for their valuable contributions to this work: Robert Pruitt for constructing the triply marked line and performing the cross with Nd-0; Mark Daly for excellent assistance with the data analysis; Stanley Tamaki, Bonnie Pruitt, and Patty Pang for assistance with plant DNA preparations; Sherry Kempin for assistance with gel blots and phage DNA preparations; and members of the laboratory of E.M.M. for comments on the manuscript. This work was supported by a National Science Foundation grant (DCB-870349) to E.M.M. J.L.B. was supported, and C.C. was partially supported, by a National Institutes of Health training grant (5T32-GM07616). E.S.L. was partially supported by a National Science Foundation grant (DCB-8611317) and by a System Development Foundation grant (G612). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
NIH Predoctoral Fellowship5T32-GM07616
System Development FoundationG612
Subject Keywords:genetic map, Brassicaceae
Issue or Number:18
PubMed Central ID:PMC282077
Record Number:CaltechAUTHORS:CHApnas88
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Official Citation:C Chang, J L Bowman, A W DeJohn, E S Lander, and E M Meyerowitz Restriction fragment length polymorphism linkage map for Arabidopsis thaliana PNAS 1988 85 (18) 6856-6860
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:657
Deposited By: Archive Administrator
Deposited On:12 Sep 2005
Last Modified:09 Mar 2020 13:18

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