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Pattern of glycosylation of sindbis virus envelope proteins synthesized in hamster and chicken cells

Mayne, Jeffrey T. and Bell, John R. and Strauss, Ellen G. and Strauss, James H. (1985) Pattern of glycosylation of sindbis virus envelope proteins synthesized in hamster and chicken cells. Virology, 142 (1). pp. 121-133. ISSN 0042-6822. http://resolver.caltech.edu/CaltechAUTHORS:20160328-153821550

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Abstract

The tryptic glycopeptides of the Sindbis virus envelope glycoproteins El and E2 grown in BHK and chick cells were purified by gel filtration followed by high-pressure liquid chromatography. Each of the purified glycopeptides was analyzed by N-terminal sequencing to identify from which of the potential glycosylation sites it was derived. The type of oligosaccharide chain attached to each glycopeptide was determined from gel filtration analysis of the pronase-digested glycopeptides, and the relative incorporation of radiolabeled galactose, mannose, and glucosamine into each glycopeptide was used to confirm these determinations. The glycosylation patterns for the two proteins were essentialJy identical in the two host cells. The E2 glycosylation sites at Asn_(196) and Asn_(318) contained exclusively complex-type and simple-type oligosaccharide chains, respectively. In El, the glycosylation site at Asn_(139) contained only complex-type chains, but the site at Asn_(245) contained a mixture of simple (75-85%) and complex (15-25%) type chains. These results are discussed in relation to previously reported results and a prediction as to the relative importance of the different glycosylation sites to the function of the proteins is made.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0042-6822(85)90427-1DOIArticle
http://www.sciencedirect.com/science/article/pii/0042682285904271PublisherArticle
Additional Information:© 1985 Academic Press, Inc. Received August 1, 1984; accepted November 27, 1984. We thank E. Lenches for the preparation of monolayers of chicken embryo fibroblasts and BHK-21 cells and the growth of large quantities of Sindbis virus, L. E. Hood for the generous loans of much of his equipment, M. W. Hunkapiller for his help and expertise with HPLC, and R.W. Compans for his advice about the purification of glycopeptides. This work was supported by Public Health Service Grants GM 06965, AI 10793, and AI 20612 from the National Institutes of Health, and Grant DMB 83 16856 from the National Science Foundation.
Funders:
Funding AgencyGrant Number
NIHGM 06965
NIHAI 10793
NIHAI 20612
NSFDMB 83-16856
Record Number:CaltechAUTHORS:20160328-153821550
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160328-153821550
Official Citation:Jeffrey T. Mayne, John R. Bell, Ellen G. Strauss, James H. Strauss, Pattern of glycosylation of sindbis virus envelope proteins synthesized in hamster and chicken cells, Virology, Volume 142, Issue 1, 1985, Pages 121-133, ISSN 0042-6822, http://dx.doi.org/10.1016/0042-6822(85)90427-1. (http://www.sciencedirect.com/science/article/pii/0042682285904271)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65718
Collection:CaltechAUTHORS
Deposited By: Donna Wrublewski
Deposited On:28 Mar 2016 23:24
Last Modified:28 Mar 2016 23:24

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