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The nonstructural proteins of Sindbis virus as studied with an antibody specific for the C terminus of the nonstructural readthrough polyprotein

López, Susana and Bell, John R. and Strauss, Ellen G. and Strauss, James H. (1985) The nonstructural proteins of Sindbis virus as studied with an antibody specific for the C terminus of the nonstructural readthrough polyprotein. Virology, 141 (2). pp. 235-247. ISSN 0042-6822. http://resolver.caltech.edu/CaltechAUTHORS:20160328-153821812

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Abstract

A dodecapeptide containing the sequence of the C terminus of the nonstructural polyprotein of Sindbis virus has been synthesized and used to immunize rabbits. The antisera obtained precipitated polypeptides from cells infected with the HR strain of Sindbis or with temperature-sensitive mutants tsll or tsl8. Four different polypeptides, having apparent molecular weights of approximately 250,000, 220,000, 155,000, and 72,000, were immunoprecipitated by the antipeptide antiserum. The largest of these polypeptides is sufficiently large to represent a polyprotein translated from the entire nonstructural region of the genome. These data suggest that nsP4 of molecular weight 72,000 is produced by translation of the entire nonstructural region of the genome, which requires readthrough of an opal termination codon immediately upstream of nsP4, followed by post-translational cleavage of this polyprotein. The amounts of nsP4 and its precursors found in infected cells are small relative to the amounts of other nonstructural proteins present, as would be expected if readthrough of a termination codon is required. In addition, the relative amounts of nsP4 and of its precursors differ in HR-infected or ts mutant-infected cells and differ with temperature of infection, suggesting that temperature of infection or ts lesions affect translation and processing of the precursor polyprotein.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0042-6822(85)90254-5DOIArticle
http://www.sciencedirect.com/science/article/pii/0042682285902545PublisherArticle
Additional Information:© 1985 Academic Press, Inc. Received August 17, 1984; accepted October 30, 1984. We are grateful to E. Lenches for expert technical assistance, to T. Hunkapiller for computer programs used in construction of the figures, to L. Hood for use of computer facilities, and to J. Shively for HF hydrolysis of peptide C. This work was supported by Grants GM06965, AI10793, and AI20612 from NIH and Grant PCM8316856 from NSF. S. L. was supported by a fellowship from the Universidad Nacíonal Autónoma de México.
Funders:
Funding AgencyGrant Number
NIHGM06965
NIHAI10793
NIHAI20612
NSFPCM 8316856
Universidad Nacional Autónoma de México (UNAM)UNSPECIFIED
Record Number:CaltechAUTHORS:20160328-153821812
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160328-153821812
Official Citation:Susana Lopez, John R. Bell, Ellen G. Strauss, James H. Strauss, The nonstructural proteins of sindbis virus as studied with an antibody specific for the C terminus of the nonstructural readthrough polyprotein, Virology, Volume 141, Issue 2, 1985, Pages 235-247, ISSN 0042-6822, http://dx.doi.org/10.1016/0042-6822(85)90254-5. (http://www.sciencedirect.com/science/article/pii/0042682285902545)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65719
Collection:CaltechAUTHORS
Deposited By: Donna Wrublewski
Deposited On:28 Mar 2016 23:50
Last Modified:28 Mar 2016 23:50

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