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Segmental lineage restrictions in the chick embryo spinal cord depend on the adjacent somites

Stern, Claudio D. and Jacques, Karen F. and Lim, Tit-Meng and Fraser, Scott E. and Keynes, Roger J. (1991) Segmental lineage restrictions in the chick embryo spinal cord depend on the adjacent somites. Development, 113 (1). pp. 239-244. ISSN 0950-1991. https://resolver.caltech.edu/CaltechAUTHORS:20160331-075736936

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Abstract

We have investigated whether the developing spinal cord is intrinsically segmented in its rostrocaudal (anteroposterior) axis by mapping the spread of clones derived from single labelled cells within the neural tube of the chick embryo. A single cell in the ventrolateral neural tube of the trunk was marked in situ with the fluorescent tracer lysinated rhodamine dextran (LRD) and its descendants located after two days of further incubation. We find that clones derived from cells labelled before overt segmentation of the adjacent mesoderm do not respect any boundaries within the neural tube. Those derived from cells marked after mesodermal segmentation, however, never cross an invisible boundary aligned with the middle of each somite, and tend to be elongated along the mediolateral axis of the neural tube. When the somite pattern is surgically disturbed, neighbouring clones derived from neuroectodermal cells labelled after somite formation behave like clones derived from younger cells: they no longer respect any boundaries, and are not elongated mediolaterally. These results indicate that periodic lineage restrictions do exist in the developing spinal cord of the chick embryo, but their maintenance requires the presence of the adjacent somite mesoderm.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dev.biologists.org/content/113/1/239PublisherArticle
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 1991 The Company of Biologists Limited. Accepted 28 May 1991. This study was funded by grants from the Medical Research Council (CDS, RJK), from the NIH to SEF and by a travel grant from the Wellcome Trust to CDS. The confocal microscope was purchased with a grant from the Medical Research Council.
Funders:
Funding AgencyGrant Number
Medical Research Council (UK)UNSPECIFIED
NIHUNSPECIFIED
Wellcome TrustUNSPECIFIED
Subject Keywords:cell lineage, segmentation, spinal cord, neural tube, neuromeres, chick embryo, somites, fluorescent dextrans
Issue or Number:1
Record Number:CaltechAUTHORS:20160331-075736936
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160331-075736936
Official Citation:Segmental lineage restrictions in the chick embryo spinal cord depend on the adjacent somites C.D. Stern, K.F. Jaques, T.M. Lim, S.E. Fraser, R.J. Keynes Development 1991 113: 239-244
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65780
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:31 Mar 2016 20:25
Last Modified:03 Oct 2019 09:50

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