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CD9, a Major Platelet Cell Surface Glycoprotein, is a ROCA Antigen and Is Expressed in the Nervous System

Kaprielian, Zaven and Cho, Kyung-Ok and Hadjiargyrou, Michael and Patterson, Paul H. (1995) CD9, a Major Platelet Cell Surface Glycoprotein, is a ROCA Antigen and Is Expressed in the Nervous System. Journal of Neuroscience, 15 (1). pp. 562-573. ISSN 0270-6474. PMCID PMC6578317. doi:10.1523/JNEUROSCI.15-01-00562.1995. https://resolver.caltech.edu/CaltechAUTHORS:20160331-085656558

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Abstract

We previously generated a monoclonal antibody (mAb), ROCA1, which binds preferentially to rostral versus caudal sympathetic ganglia and intercostal nerves. Two other mAbs, ROCA2 and B2C11, bind to the same structures but not in rostrocaudal gradients. All three mAbs recognize a 26 kDa cell surface protein. Amino acid sequence data obtained from the affinity purified 26 kDa protein showed some homology with human CD9, a tetraspan protein implicated in intercellular signaling in hematopoietic cells. Using the PCR, we obtained cDNA clones representing the entire rat CD9 coding sequence from sciatic nerve and sympathetic ganglia. ROCA1, ROCA2, and B2C11 each immunoprecipitate a 26 kDa protein from CHO cells stably transfected with one of the clones, demonstrating that the ROCA cell surface antigen is indeed rat CD9. We find that CD9 mRNA is widely expressed, with particularly high levels present in a number of neural tissues. In situ hybridization demonstrates that peripheral neurons and Schwann cells, as well as adrenal chromaffin cells express CD9 mRNA. Consistent with immunoblot analyses showing that, unlike the ROCA1 epitope, the 26 kDa protein is not expressed in a rostrocaudal gradient, we find similar levels of rat CD9 mRNA in rostral and caudal intercostal nerves. In developing postnatal rat sciatic nerve, CD9 mRNA levels are coordinately regulated with the expression of myelin genes. These results provide another example of a cell surface protein expressed by both hematopoietic and neural cells, and suggest a role for CD9 in intercellular signaling in the nervous system.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1523/JNEUROSCI.15-01-00562.1995DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6578317PubMed CentralArticle
Additional Information:© 1995 Society for Neuroscience. For the first six months after publication SfN’s license will be exclusive. Beginning six months after publication the Work will be made freely available to the public on SfN’s website to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/). Received Mar. 29, 1994; revised June 13, 1994; accepted July 12, 1994. We thank Dr. Richard Akeson for mAb B2C11; Doreen McDowell for assistance in platelet and media preparation; Li Ching Lo for assistance in in situ hybridization; Lisa Banner, Ming Ji Fann, and Kai Zinn for help with primer selection and PCR analysis; and Joshua Sanes, Karen Allendorfer, and Shilpi Banerjee for critically reading the manuscript. This work was supported by an individual NRSA to Z.K., an American Heart Association Research Training fellowship to M.H., and a NINDS grant to P.H.P.
Funders:
Funding AgencyGrant Number
American Heart AssociationUNSPECIFIED
National Institute of Neurological Disorders and Stroke (NINDS)UNSPECIFIED
NIH Predoctoral FellowshipUNSPECIFIED
Subject Keywords:CD9, tetraspan family, ROCA antigen, neurospecification, Schwann cell, platelets, adrenal chromaffin cells, myelin, cell surface
Issue or Number:1
PubMed Central ID:PMC6578317
DOI:10.1523/JNEUROSCI.15-01-00562.1995
Record Number:CaltechAUTHORS:20160331-085656558
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160331-085656558
Official Citation:CD9, a major platelet cell surface glycoprotein, is a ROCA antigen and is expressed in the nervous system. Z Kaprielian, KO Cho, M Hadjiargyrou, PH Patterson. Journal of Neuroscience 1 January 1995, 15 (1) 562-573; DOI: 10.1523/JNEUROSCI.15-01-00562.1995
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65788
Collection:CaltechAUTHORS
Deposited By:INVALID USER
Deposited On:31 Mar 2016 20:03
Last Modified:17 Feb 2022 00:30

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