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Comparison of the Hindin Proteins of Strongylocentrotus franciscanus, S. purpuratus, and Lytechinus variegatus: Sequences involved in the Species Specificity of Fertilization

Minor, Joseph E. and Fromson, David R. and Britten, Roy J. and Davidson, Eric H. (1991) Comparison of the Hindin Proteins of Strongylocentrotus franciscanus, S. purpuratus, and Lytechinus variegatus: Sequences involved in the Species Specificity of Fertilization. Molecular Biology and Evolution, 8 (6). pp. 781-795. ISSN 0737-4038. https://resolver.caltech.edu/CaltechAUTHORS:20160331-111921056

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Abstract

Bindin is the sea urchin sperm acrosomal protein that is responsible for the species-specific adhesion of the sperm to the egg. Two new bindin cDNA sequences that contain the entire open reading frame for the binding precursor are reported: one for Strongylocentrotus franciscanus and one for Lytechinus variegatus. Both contain inverted repetitive sequences in their 3' untranslated regions, and the S. franciscanus cDNA contains an inverted repetitive sequence match between the 5' untranslated region and the coding region. The middle third of the mature bindin sequence is highly conserved in all three species, and the flanking sequences share short repeated sequences that vary in number between the species. Cross-fertilization data are reported for the species S. purpuratus, S. franciscanus, L. variegatus, and L. pictus. A barrier to cross-fertilization exists between the sympatric Strongylocentrotus species, but there is no barrier between the allopatric Lytechinus species.


Item Type:Article
Related URLs:
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http://mbe.oxfordjournals.org/content/8/6/781.abstractPublisherArticle
Additional Information:© 1991 The University of Chicago. Received January 23, 1991; revision received May 7, 1991; Accepted May 7, 1991. Richard K. Koehn, reviewing editor. We thank Roger Anderson, Dr. D. Nishioka, and the two reviewers for helpful comments on the manuscript. We also thank Ors. F. Calzone, S. Fain, H. Hwu, S. Kim, D. Livant, F. Preugschat, and M. J. Smith and S. Westaway for genomic DNAs used in the zoo blots. This work was supported by NSF grant DCB-8813413.
Funders:
Funding AgencyGrant Number
NSFDCB-8813413
Subject Keywords:fertilization, bindin, species specificity, inverted repeats, echinoderm
Issue or Number:6
Record Number:CaltechAUTHORS:20160331-111921056
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160331-111921056
Official Citation:J E Minor, D R Fromson, R J Britten, and E H Davidson Comparison of the bindin proteins of Strongylocentrotus franciscanus, S. purpuratus, and Lytechinus variegatus: sequences involved in the species specificity of fertilization. Mol Biol Evol (1991) 8 (6): 781-795
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65804
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:31 Mar 2016 19:39
Last Modified:03 Oct 2019 09:50

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