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Placodal Origin of Brn-3—Expressing Cranial Sensory Neurons

Artinger, Kristin B. and Fedtsova, Natalia and Rhee, Jerry M. and Bronner-Fraser, Marianne and Turner, Eric (1998) Placodal Origin of Brn-3—Expressing Cranial Sensory Neurons. Journal of Neurobiology, 36 (4). pp. 572-585. ISSN 0022-3034. http://resolver.caltech.edu/CaltechAUTHORS:20160331-142049577

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Abstract

The Brn-3 class of POU-domain transcription factors includes three genes in mammals which have key roles in the development of specific groups of sensory neurons. Here, we have identified three avian genes which correspond to the murine genes Brn-3.0, Brn-3.1, and Brn-3.2. Using an in situ hybridization probe generic for this gene class, the earliest detectable expression of Brn-3 in the chick is at stage 15, in placodal and migrating precursors of the trigeminal ganglion. By stage 19, Brn-3.0 protein is detectable in the trigeminal and vestibulocochlear ganglia with Brn-3.0-specific antisera, and Brn-3 message expression has extended to the dorsal root ganglia. At later stages, when condensation of the trigeminal ganglion is complete, Brn-3.0-immunoreactive neurons are concentrated in the portion of the ganglion distal to the brain stem. To examine the developmental origin of the Brn-3 expressing cells, we combined lipophilic dye (DiI) labeling with in situ hybridization. DiI labeling of the placodal surface ectoderm and of premigratory neural crest cells in the neural tube reveals that all, or nearly all, of the Brn-3-expressing neurons in the trigeminal ganglia are derived from the sensory placodes and not from the neural crest, and thus, that Brn-3 is an early marker of the placode-derived sensory neural lineage.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/(SICI)1097-4695(19980915)36:4<572::AID-NEU10>3.0.CO;2-ADOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/%28SICI%291097-4695%2819980915%2936:4%3C572::AID-NEU10%3E3.0.CO;2-A/abstractPublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1998 John Wiley & Sons, Inc. Received 5 March 1998; accepted 4 May 1998. Tue authors are indebted to Dr. John Sechrist for his participation in the experiments and extensive review of the manuscript. Eric E. Turner and Natalia Fedtsova are NARSAD Young Investigators, and NF was supported in part by NIH Training Grant. 5-T32-MH19934. This work was suppo11ed in part by the Pfizer New Faculty Award, Scottish Rite Schizophrenia Research Program, Department of Veterans Affairs MERIT funding, and USPHS awards HD33442 and MH58447 to EET and USPHS awards NS36585 and NS34671 to MBF.
Funders:
Funding AgencyGrant Number
NIH5-T32-MH19934
PfizerUNSPECIFIED
Scottish Rite Schizophrenia Research ProgramUNSPECIFIED
Department of Veterans AffairsUNSPECIFIED
U.S. Public Health Service (USPHS)HD33442
U.S. Public Health Service (USPHS)MH58447
NIHNS36585
NIHNS34671
National Alliance for Research on Schizophrenia and Depression (NARSAD)UNSPECIFIED
Subject Keywords:Brn-3; sensory placode; trigeminal ganglion; POU domain; neural crest
Record Number:CaltechAUTHORS:20160331-142049577
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160331-142049577
Official Citation:Artinger, K. B., Fedtsova, N., Rhee, J. M., Bronner-Fraser, M. and Turner, E. (1998), Placodal origin of Brn-3—expressing cranial sensory neurons. J. Neurobiol., 36: 572–585. doi: 10.1002/(SICI)1097-4695(19980915)36:4<572::AID-NEU10>3.0.CO;2-A
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65814
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:31 Mar 2016 22:25
Last Modified:31 Mar 2016 22:25

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