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Regionalization of the Developing Forebrain: A Comparison of FORSE-1, Dlx-2, and BF-1

Tole, Shubha and Patterson, Paul H. (1995) Regionalization of the Developing Forebrain: A Comparison of FORSE-1, Dlx-2, and BF-1. Journal of Neuroscience, 15 (2). pp. 970-980. ISSN 0270-6474. PMCID PMC6577806. doi:10.1523/JNEUROSCI.15-02-00970.1995.

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The FORSE-1 monoclonal antibody (mAb) was generated using a strategy designed to produce mAbs against neuronal cell surface antigens that might be regulated by regionally restricted transcription factors in the developing CNS. To determine whether FORSE-1 has a labeling pattern similar to that of known transcription factors, the expression of BF-1 and Dlx-2 was examined by in situ hybridization on sections serial to those labeled with FORSE-1. We find a striking overlap between BF-1 and FORSE-1 in the telencephalon; both are expressed in the lateral but not the medial walls of the telencephalon, and the boundaries of expression are apparently identical. FORSE-1 staining is detected prior to BF-1 expression in the neural tube, however. FORSE-1 and Dlx-2 have very different patterns of expression in the forebrain, suggesting that regulation by Dlx-2 cannot by itself explain the distribution of FORSE- 1. However, they share some sharp boundaries in the diencephalon. In addition, FORSE-1 identifies some previously unknown boundaries in the developing forebrain. These results indicate that a new cell surface marker can be used to subdivide the embryonic telencephalon and diencephalon into regions smaller than previously described, providing necessary complexity to the developmental patterning in the forebrain.

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Additional Information:© 1995 by Society for Neuroscience. Beginning six months after publication the Work will be made freely available to the public on SfN’s website to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license ( Received May 3, 1994; revised July 18, 1994; accepted July 25, 1994. We thank Dr. John Rubenstein for the probe to Dlx-2; Dr. Eseng Lai for the probe to BF-I; Dr. Salvadore Martinez for useful discussions; and Drs. Karen Allendoerfer, Scott Fraser, and Z. Kaprielian for helpful comments on the manuscript. We also thank Bob Turing, Richard Gomez, Ben Sewell, Lance Brown, Ella McClanahan, Jim Staub, Theo Steiner, Alice Edel, and Le Hanh Dinh from the Graphic Arts facility at Caltech for their assistance with the illustrations. This work was supported by an NINDS grant to P.H.P.
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National Institute of Neurological Disorders and Stroke (NINDS)UNSPECIFIED
Issue or Number:2
PubMed Central ID:PMC6577806
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Official Citation:Regionalization of the developing forebrain: a comparison of FORSE-1, Dlx-2, and BF-1. S Tole, PH Patterson. Journal of Neuroscience 1 February 1995, 15 (2) 970-980; DOI: 10.1523/JNEUROSCI.15-02-00970.1995
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65827
Deposited By: Ruth Sustaita
Deposited On:01 Apr 2016 20:03
Last Modified:17 Feb 2022 00:30

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