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Developing integrated single-cell metabolic/proteomic assays

Xue, Min and Wei, Wei and Su, Yapeng and Shin, Young Shik and Kim, Jungwoo and Heath, James R. (2015) Developing integrated single-cell metabolic/proteomic assays. Cancer Research, 75 (S). Art. No. 1995. ISSN 0008-5472. https://resolver.caltech.edu/CaltechAUTHORS:20160401-090255899

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Abstract

The technology boom of single-cell metabolomics and proteomics has led to exciting biological discoveries in the past decade. It has become widely acknowledged that resolving the intratumoral heterogeneity at single cell resolution can aid the understanding of cancer therapeutic response and progression, as well as facilitate the development of therapeutic interventions. Here we present an integrated assay that simultaneously quantifies the intracellular metabolites, glucose uptake, metabolic proteins and functional signaling proteins at the single-cell level. The measurement of metabolites is achieved through a series of competitive binding assays and is incorporated into the microfluidic-based single cell barcode chip platform. We demonstrate that this assay is capable of obtaining information from both protein and metabolic signaling pathways associated with tumor survival and progression, providing new insights into cancer heterogeneity and drug responsiveness.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1158/1538-7445.AM2015-1995DOIArticle
http://cancerres.aacrjournals.org/content/75/15_Supplement/1995PublisherArticle
ORCID:
AuthorORCID
Kim, Jungwoo0000-0002-5215-2044
Heath, James R.0000-0001-5356-4385
Additional Information:© 2015 American Association for Cancer Research.
Issue or Number:S
Record Number:CaltechAUTHORS:20160401-090255899
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160401-090255899
Official Citation:Molecular and Cellular Biology - Poster Presentations - Proffered Abstracts - Poster Presentations - New Developments in Proteomics: Abstract 1995: Developing integrated single-cell metabolic/proteomic assays Min Xue, Wei Wei, Yapeng Su, Young Shik Shin, Jungwoo Kim, and James R. Heath Cancer Res August 1, 2015 75:1995; doi:10.1158/1538-7445.AM2015-1995
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65839
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:01 Apr 2016 20:00
Last Modified:03 Oct 2019 09:50

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