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Phylogenetic Relationships of Reverse Transcriptase and RNase H Sequences and Aspects of Genome Structure in the Gypsy Group of Retrotransposons

Springer, Mark S. and Britten, Roy J. (1993) Phylogenetic Relationships of Reverse Transcriptase and RNase H Sequences and Aspects of Genome Structure in the Gypsy Group of Retrotransposons. Molecular Biology and Evolution, 10 (6). pp. 1370-1379. ISSN 0737-4038. https://resolver.caltech.edu/CaltechAUTHORS:20160401-113735010

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Abstract

The gypsy group of long-terminal-repeat retrotransposons contains elements having the same order of enzyme domains in the pol gene as do retroviruses. Elements in the gypsy group are now known from yeast, filamentous fungi, plants, insects, and echinoids. Reverse transcriptase and RNase H amino acid sequences from elements in the gypsy group--including the recently described SURL elements, TED, Cft1, and Ulysses,--were aligned and analyzed by using parsimony and bootstrapping methods, with plant caulimoviruses and/or retroviruses as outgroups. Clades supported at the 95% level after bootstrapping include (1) 17.6 with 297 and (2) all of the SURL elements together. Other likely relationships supported at lower bootstrap confidence intervals include (1) SURL elements with mag, (2) 17.6 and 297 with TED, and this collective group with 412 and gypsy, (3) Tf1 with Cft1, (4) IFG7 with Del, and (5) all of the retrotransposons in the gypsy group together, to the exclusion of Ty3. In contrast with an earlier analysis, our results place mag within the gypsy group rather than outside of a cluster that contains gypsy group retrotransposons and plant caulimoviruses. Several features of retrotransposon genomes provide further support for some of the aforementioned relationships. The union of SURL elements with mag is supported by the presence of two RNA binding sites in the nucleocapsid protein. Location of the tRNA primer binding site and the presence of a long open reading frame 3' to the pol gene support the 17.6-297-TED-412-gypsy cluster.


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http://mbe.oxfordjournals.org/content/10/6/1370.abstractPublisherArticle
Additional Information:© 1993 University of Chicago. Received January 6, 1993; revision received July 5, 1993; Accepted July 23, 1993. We would like to thank Walter Fitch and two anonymous reviewers for helpful comments. Walter M. Fitch, reviewing editor.
Subject Keywords:retrotransposon, reverse transcriptase, RNase H
Issue or Number:6
Record Number:CaltechAUTHORS:20160401-113735010
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160401-113735010
Official Citation:M S Springer and R J Britten Phylogenetic relationships of reverse transcriptase and RNase H sequences and aspects of genome structure in the gypsy group of retrotransposons. Mol Biol Evol (1993) 10 (6): 1370-1379
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65852
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:01 Apr 2016 19:54
Last Modified:03 Oct 2019 09:51

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