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Functional Expression of the Heteromeric “Olfactory” Cyclic Nucleotide-Gated Channel in the Hippocampus: A Potential Effector of Synaptic Plasticity in Brain Neurons

Bradley, Jonathan and Zhang, Yinong and Bakin, Robert and Lester, Henry A. and Ronnett, Gabriele V. and Zinn, Kai (1997) Functional Expression of the Heteromeric “Olfactory” Cyclic Nucleotide-Gated Channel in the Hippocampus: A Potential Effector of Synaptic Plasticity in Brain Neurons. Journal of Neuroscience, 17 (6). pp. 1993-2005. ISSN 0270-6474. PMCID PMC6793760. https://resolver.caltech.edu/CaltechAUTHORS:20160401-135631881

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Abstract

Cyclic nucleotide-gated (cng) channels are important components of signaling systems mediating sensory transduction. In vertebrate photoreceptors, light activates a signaling cascade that causes a decrease in intracellular cGMP concentrations, closing retinal cng channels. Signal transduction in olfactory receptor neurons is believed to proceed via G-protein-mediated elevation of intracellular cAMP in response to odorant binding by 7-helix receptors. cAMP opens the olfactory cng channel, which is highly permeable to Ca^(2+). Here we demonstrate by in situ hybridization and immunohistochemistry with subunit-specific antibodies that both subunits of the heteromeric rat olfactory cng channel are also widely expressed in the brain. Expression of the retinal rod cng channel, however, can be detected only in the eye. In the adult hippocampus, the olfactory cng channel is expressed on cell bodies and processes of CA1 and CA3 neurons. In cultured embryonic hippocampal neurons, the channel is localized to a subset of growth cones and processes. We recorded conductances with the electrophysiological characteristics of the heteromeric olfactory cng channel in excised inside-out patches from these cultured neurons. We also show that Ca^(2+) influx into hippocampal neurons in response to cyclic nucleotide elevation can be detected using fura-2 imaging. Cyclic nucleotide elevation has been implicated in several mechanisms of synaptic plasticity in the hippocampus, and these mechanisms also require elevation of intracellular Ca^(2+). Our results suggest that the “olfactory” cng channel could regulate synaptic efficacy in brain neurons by modulating Ca^(2+) levels in response to changes in cyclic nucleotide concentrations.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1523/JNEUROSCI.17-06-01993.1997DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6793760PubMed CentralArticle
ORCID:
AuthorORCID
Lester, Henry A.0000-0002-5470-5255
Zinn, Kai0000-0002-6706-5605
Additional Information:© 1997 Society for Neuroscience. Beginning six months after publication the Work will be made freely available to the public on SfN’s website to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/). Received Nov. 11, 1996; revised Dec. 23, 1996; accepted Dec. 30, 1996. This work was supported by a grant from the National Institutes of Mental Health to K.Z., and by grants from the National Institute of Deafness and Communication Disorders and the W. M. Keck Foundation to G.R. J.B. was supported by a National Institutes of Health (NIH) graduate training grant and Awards for Research College Scientists Foundation. Y.Z. was supported by a National Research Service Award postdoctoral fellowship from NIH. We thank Norman Davidson, Mary Kennedy, Jun Li, Ming-Ji Fann, Chris Schoenherr, Yasuhito Uezono, David Anderson, Cori Bargmann, and members of the Zinn, Ronnett, Lester, Davidson, Anderson, and Kennedy groups for helpful discussions; Susan Ou and the Caltech Monoclonal Antibody Facility for generation of hybridomas; and Sheri McKinney for hippocampal cultures.
Funders:
Funding AgencyGrant Number
National Institute of Mental Health (NIMH)UNSPECIFIED
National Institute on Deafness and Other Communication DisordersUNSPECIFIED
W. M. Keck FoundationUNSPECIFIED
NIHUNSPECIFIED
Research College Scientists FoundationUNSPECIFIED
Subject Keywords:cyclic nucleotide-gated channels cAMP cGMP olfaction sensory transduction hippocampus synaptic plasticity
Issue or Number:6
PubMed Central ID:PMC6793760
Record Number:CaltechAUTHORS:20160401-135631881
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160401-135631881
Official Citation: Functional Expression of the Heteromeric “Olfactory” Cyclic Nucleotide-Gated Channel in the Hippocampus: A Potential Effector of Synaptic Plasticity in Brain Neurons. Jonathan Bradley, Yinong Zhang, Robert Bakin, Henry A. Lester, Gabriele V. Ronnett, Kai Zinn. Journal of Neuroscience 15 March 1997, 17 (6) 1993-2005; DOI: 10.1523/JNEUROSCI.17-06-01993.1997
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65857
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:01 Apr 2016 22:26
Last Modified:28 Oct 2019 16:57

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