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Intersecting batteries of differentially expressed genes in the early sea urchin embryo

Thiebaud, Pierre and Goodstein, Marcia and Calzone, Frank J. and Thézé, Nadine and Britten, Roy J. and Davidson, Eric H. (1990) Intersecting batteries of differentially expressed genes in the early sea urchin embryo. Genes and Development, 4 (11). pp. 1999-2010. ISSN 0890-9369. doi:10.1101/gad.4.11.1999.

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We determined the distribution of cis-regulatory sites, previously identified in the control domain of the CyIIIa gene, in three other genes displaying diverse spatial patterns of expression in the sea urchin embryo. Competitive gel-shift reactions were carried out using probes from the CyIIIa gene, with competitor fragments isolated from the previously defined control domains of the other genes. CyIIIa is expressed only in aboral ectoderm lineages; the other genes studied were Spec1, also expressed in aboral ectoderm; CyI, expressed in many different cell types; and SM50, expressed only in skeletogenic mesenchyme. All four genes are activated at about the same time in late cleavage. Where competitive interactions indicated a functionally comparable binding site (in vitro), a sequence homology was sought, and in most cases could be identified. An interesting pattern of putative regulatory site usage emerges: Of 10 CyIIIa interactions tested, three only were unique to the CyIIIa gene with respect to the set of four genes tested; one believed on previous evidence to be a temporal regulator was shared by all four genes, and the remainder were shared in various subsets of the four genes.

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Additional Information:© 1999 Cold Spring Harbor Laboratory Press. The Authors acknowledge that six months after the full-issue publication date, the Article will be distributed under a Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License, Received June 20, 1990; revised version accepted August 17, 1990. This research was supported by National Institutes of Health grant HD-05753. P.T. was partially supported by a CNRS/NSF Fellowship and MRE (Ministere des relations exterieures). F.J.C. was partially supported by a grant from the American Cancer Society (IRG IN166B). The publication costs of this article were defrayed in part by payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.
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Centre National de la Recherche Scientifique (CNRS)UNSPECIFIED
Ministere des Relations Exterieures (MRE)UNSPECIFIED
American Cancer SocietyIRG IN 166B
Subject Keywords:Development; sea urchin embryos; gene batteries; cis-regulatory sites
Issue or Number:11
Record Number:CaltechAUTHORS:20160401-142756314
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Official Citation:Intersecting batteries of differentially expressed genes in the early sea urchin embryo. P Thiebaud, M Goodstein, F J Calzone, N Thézé, R J Britten, and E H Davidson Genes Dev. November 1990 4: 1999-2010; doi:10.1101/gad.4.11.1999
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65858
Deposited By: Ruth Sustaita
Deposited On:01 Apr 2016 22:30
Last Modified:10 Nov 2021 23:50

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