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An Antibody to the Tetraspan Membrane Protein CD9 Promotes Neurite Formation in a Partially α3β1 Integrin-Dependent Manner

Banerjee, Shilpi A. and Hadjiargyrou, Michael and Patterson, Paul H. (1997) An Antibody to the Tetraspan Membrane Protein CD9 Promotes Neurite Formation in a Partially α3β1 Integrin-Dependent Manner. Journal of Neuroscience, 17 (8). pp. 2756-2765. ISSN 0270-6474. PMCID PMC6573113. https://resolver.caltech.edu/CaltechAUTHORS:20160404-064903976

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Abstract

The tetraspan cell surface glycoprotein, CD9, has been implicated in cellular signaling during growth and differentiation in the hematopoietic and nervous systems. Because CD9 expression is induced early in development in sensory and sympathetic neuroblasts, we investigated the role of CD9 in neurite outgrowth. We plated dissociated cells from neonatal sympathetic ganglia on immobilized anti-CD9 antibodies or antibodies against other cell surface molecules. We show here that B2C11, an anti-CD9 antibody that has been shown previously to activate Schwann cells in vitro, promotes robust neurite outgrowth from sympathetic neurons that is greater than that on other antibody surfaces and is comparable to neurite outgrowth on a collagen substratum. In addition, B2C11 causes dramatic morphological changes in neurons and glia from dissociated ganglia, including a flattening of these cells. Because CD9 interacts with integrins in many cell types including Schwann cells, and specifically with the α3β1 integrin in some cells, we tested whether the effect of B2C11 on neurite outgrowth is mediated by this integrin. An anti-α3β1 antibody, Ralph 3–1, attenuates the extent of neurite outgrowth on B2C11 and collagen, but not on laminin. Because the α3β1 integrin has been shown to mediate neurite outgrowth on different substrates, these results provide a functional significance for the CD9-α3β1 interaction; downstream signaling may be activated by this cis interaction on the cell surface in response to external cues that promote neurite outgrowth.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1523/JNEUROSCI.17-08-02756.1997DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6573113PubMed CentralArticle
Additional Information:© 1997 Society for Neuroscience. For the first six months after publication SfN’s license will be exclusive. Beginning six months after publication the Work will be made freely available to the public on SfN’s website to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/). Received Nov. 18, 1996; revised Jan. 24, 1997; accepted Jan. 28, 1997. This work was supported by an NINDS grant to P.H.P., a Muscular Dystrophy Association Research Fellowship to S.A.B., and an National Institutes of Health training grant to M.H. We are grateful to Louis Reichardt (University of California, San Francisco) for the gift of the Ralph 3–1 mAb and to Urs Rutishauser (Case Western Reserve University, Cleveland) for the NCAM mAbs. We thank Doreen McDowell for media preparation and Karen Allendoerfer, Lisa Banner, Flora De Pablo, and Reto Gadient for providing comments on this manuscript.
Funders:
Funding AgencyGrant Number
National Institute of Neurological Disorders and Stroke (NINDS)UNSPECIFIED
Muscular Dystrophy AssociationUNSPECIFIED
NIHUNSPECIFIED
Subject Keywords:CD9; tetraspan proteins; antibody perturbation; neurite outgrowth; sympathetic neurons; a3b1 integrin
Issue or Number:8
PubMed Central ID:PMC6573113
Record Number:CaltechAUTHORS:20160404-064903976
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160404-064903976
Official Citation:An Antibody to the Tetraspan Membrane Protein CD9 Promotes Neurite Formation in a Partially α3β1 Integrin-Dependent Manner. Shilpi A. Banerjee, Michael Hadjiargyrou, Paul H. Patterson. Journal of Neuroscience 15 April 1997, 17 (8) 2756-2765; DOI: 10.1523/JNEUROSCI.17-08-02756.1997
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65868
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:06 Apr 2016 22:10
Last Modified:28 Oct 2019 16:58

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