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Isolation of the Progenitor Cells of the Sympathoadrenal Lineage from Embryonic Sympathetic Ganglia with the SA Monoclonal Antibodies

Carnahan, Josette F. and Patterson, Paul H. (1991) Isolation of the Progenitor Cells of the Sympathoadrenal Lineage from Embryonic Sympathetic Ganglia with the SA Monoclonal Antibodies. Journal of Neuroscience, 11 (11). pp. 3520-3530. ISSN 0270-6474. PMCID PMC6575542. https://resolver.caltech.edu/CaltechAUTHORS:20160404-073836354

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Abstract

Our previous articles in this series described the production of five monoclonal antibodies (SA1-5) that bind to adrenal chromaffin cells and to cells in embryonic sympathetic ganglia and adrenal primordia (Carnahan and Patterson, 1991), and the downregulation of the sympathoadrenal (SA) antigens in vivo as neuronal markers begin to be expressed (Anderson et al., 1991). These results support the hypothesis that sympathetic neurons and adrenal chromaffin cells are derived from a common embryonic progenitor that displays both neuron- and chromaffin cell-specific markers. We have taken advantage of the fact that at least some of the SA antigens are expressed on the cell surface to isolate SA+ cells from embryonic day 14.5 rat superior cervical, sympathetic ganglia by fluorescence-activated cell sorting. This population of cells is significantly enriched in the expression of markers (tyrosine hydroxylase and neurofilament) found in the putative progenitors in situ. Growth in glucocorticoid maintains the expression of the SA antigens in the sorted cells and induces the chromaffin cell marker enzyme phenylethanolamine N-methyl transferase. In contrast, growth of the sorted cells in basic fibroblast growth factor, NGF, and insulin results in the rapid loss of SA 1 expression and the outgrowth of neurites. The ability to manipulate the fate of the SA+ cells in vitro confirms the suggestion from the in vivo observations that the SA+ cells in the ganglia are at least bipotential progenitors, capable of differentiating along the chromaffin or neuronal pathways.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1523/JNEUROSCI.11-11-03520.1991DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6575542PubMed CentralArticle
Additional Information:© 1991 by Society for Neuroscience. For the first six months after publication SfN’s license will be exclusive. Beginning six months after publication the Work will be made freely available to the public on SfN’s website to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/). Received Jan. 25, 1991; revised May 1, 1991; accepted June 11, 1991. This work was supported by the NINDS (Javits Neuroscience Investigator Award) and a McKnight Foundation Neuroscience Research Project Award. We thank David Anderson for constructive comments on the experiments and on the manuscript, Doreen McDowell for help with tissue culture materials, To Ha Thai and Susan Ou for help with monoclonal antibody preparation, and Shelly Diamond and Pat Koen for help with the cell sorting experiments.
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Funding AgencyGrant Number
National Institute of Neurological Disorders and Stroke (NINDS)UNSPECIFIED
McKnight FoundationUNSPECIFIED
NIHUNSPECIFIED
Issue or Number:11
PubMed Central ID:PMC6575542
Record Number:CaltechAUTHORS:20160404-073836354
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160404-073836354
Official Citation:Isolation of the progenitor cells of the sympathoadrenal lineage from embryonic sympathetic ganglia with the SA monoclonal antibodies. JF Carnahan, PH Patterson. Journal of Neuroscience 1 November 1991, 11 (11) 3520-3530; DOI: 10.1523/JNEUROSCI.11-11-03520.1991
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65871
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:06 Apr 2016 22:02
Last Modified:30 Oct 2019 17:23

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