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Molecular Cloning and DNA Sequence of the Arabidopsis thaliana Alcohol Dehydrogenase Gene

Chang, Caren and Meyerowitz, Elliot M. (1986) Molecular Cloning and DNA Sequence of the Arabidopsis thaliana Alcohol Dehydrogenase Gene. Proceedings of the National Academy of Sciences of the United States of America, 83 (5). pp. 1408-1412. ISSN 0027-8424. PMCID PMC323085.

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Arabidopsis thaliana provides an excellent experimental plant system for molecular genetics because of its remarkably small genome size, near absence of dispersed middle repetitive DNA, and short life cycle. We have cloned and determined the nucleotide sequence of a single-copy gene from A. thaliana likely to be the gene encoding alcohol dehydrogenase (ADH; alcohol:NAD+ oxidoreductase, EC The gene was isolated from a random recombinant library by cross-hybridization with a maize Adh1 gene probe. The DNA sequence contains an open reading frame capable of encoding a polypeptide the same length as maize ADH1 and ADH2 (379 amino acids) and having approx 80% homology with both maize enzymes. This open reading frame is interrupted by six introns whose positions are conserved with six of the nine intron positions present in both maize genes. The 5' and 3' untranslated regions are, respectively, 58 and 204 base pairs long. Sequences important for eukaryotic gene expression such as the TATA box, polyadenylylation signal, and intron splicesite sequences are found in the expected locations. The gene hybridizes to a specific anaerobically induced RNA in Arabidopsis whose appearance correlates with the anaerobic induction of Arabidopsis ADH protein.

Item Type:Article
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URLURL TypeDescription CentralArticle
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1986 by the National Academy of Sciences. Communicated by Ray D. Owen, October 18, 1985. We are grateful to C. Rice for technical advice and to T. Hunkapiller and C. Martin forDNA sequence analysis programs. We thank M. Garfinkel, P. Mathers, K. Fryxell, and R. Pruitt for comments on the manuscript. This work was supported by Grant PCM-8408504 from the National Science Foundation (E.M.M.). C.C. was supported by National Research Service Award 5 T32GM07616 from the National Institutes of Health. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
NIH Predoctoral Fellowship5 T32GM07616
Subject Keywords:gene structure, enzyme Induction, intron, evolution
Issue or Number:5
PubMed Central ID:PMC323085
Record Number:CaltechAUTHORS:CHApnas86
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Official Citation:C Chang and E M Meyerowitz Molecular cloning and DNA sequence of the Arabidopsis thaliana alcohol dehydrogenase gene PNAS 1986 83 (5) 1408-1412
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:659
Deposited By: Archive Administrator
Deposited On:12 Sep 2005
Last Modified:02 Oct 2019 22:35

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