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Polypeptide requirements for assembly of functional Sindbis virus replication complexes: a model for the temporal regulation of minus- and plus-strand RNA synthesis

Lemm, Julie A. and Rümenapf, Tillmann and Strauss, Ellen G. and Strauss, James H. and Rice, Charles M. (1994) Polypeptide requirements for assembly of functional Sindbis virus replication complexes: a model for the temporal regulation of minus- and plus-strand RNA synthesis. EMBO Journal, 13 (12). pp. 2925-2934. ISSN 0261-4189. PMCID PMC395174. https://resolver.caltech.edu/CaltechAUTHORS:20160404-161700052

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Abstract

Proteolytic processing of the Sindbis virus non-structural polyproteins (P123 and P1234) and synthesis of minus- and plus-strand RNAs are highly regulated during virus infection. Although their precise roles have not been defined, these polyproteins, processing intermediates or mature cleavage products (nsP1-4) are believed to be essential components of viral replication and transcription complexes. In this study, we have shown that nsP4 can function as the polymerase for both minus- and plus-strand RNA synthesis. Mutations inactivating the nsP2 proteinase, resulting in uncleaved P123, led to enhanced accumulation of minus-strand RNAs and reduced accumulation of genomic and subgenomic plus-strand RNAs. In contrast, no RNA synthesis was observed with a mutation which increased the efficiency of P123 processing. Inclusion of this mutation in a P123 polyprotein with cleavage sites 1/2 and 2/3 blocked allowed synthesis of both minus- and plus-strand RNAs. We conclude that nsP4 and uncleaved P123 normally function as the minus-strand replication complex, and propose that processing of P123 switches the template preference of the complex to minus-strands, resulting in efficient synthesis of plus-strand genomic and subgenomic RNAs and shut-off of minus-strand RNA synthesis.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC395174/PubMed CentralArticle
Additional Information:© Oxford University Press Received on March 8, 1994; revised on April 7, 1994 We thank our colleagues for helpful discussions during the course of this work and Ors Dorothea Sawicki, Sondra Schlesinger and Yukio Shirako for critical reading of the manuscript. This work was supported by grants from the Public Health Service (AI24134, AIl0793).
Funders:
Funding AgencyGrant Number
NIHAI 24134
NIHAI 10793
Issue or Number:12
PubMed Central ID:PMC395174
Record Number:CaltechAUTHORS:20160404-161700052
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160404-161700052
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65911
Collection:CaltechAUTHORS
Deposited By: Donna Wrublewski
Deposited On:05 Apr 2016 16:45
Last Modified:03 Oct 2019 09:51

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