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The genesis of avian neural crest cells: A classic embryonic induction

Selleck, Mark A. J. and Bronner-Fraser, Marianne (1996) The genesis of avian neural crest cells: A classic embryonic induction. Proceedings of the National Academy of Sciences of the United States of America, 93 (18). pp. 9352-9357. ISSN 0027-8424. PMCID PMC38431. https://resolver.caltech.edu/CaltechAUTHORS:20160405-100819036

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Abstract

Neural crest cells arise from the ectoderm and are first recognizable as discrete cells in the chicken embryo when they emerge from the neural tube. Despite the classical view that neural crest precursors are a distinct population lying between epidermis and neuroepithelium, our results demonstrate that they are not a segregated population. Cell lineage analyses have demonstrated that individual precursor cells within the neural folds can give rise to epidermal, neural crest, and neural tube derivatives. Interactions between the neural plate and epidermis can generate neural crest cells, since juxtaposition of these tissues at early stages results in the formation of neural crest cells at the interface. Inductive interactions between the epidermis and neural plate can also result in "dorsalization" of the neural plate, as assayed by the expression of the Wnt transcripts characteristic of the dorsal neural tube. The competence of the neural plate changes with time, however, such that interaction of early neural plate with epidermis generates only neural crest cells, whereas interaction of slightly older neural plate with epidermis generates neural crest cells and Wnt-expressing cells. At cranial levels, neuroepithelial cells can regulate to generate neural crest cells when the endogenous neural folds are removed, probably via interaction of the remaining neural tube with the epidermis. Taken together, these experiments demonstrate that: (i) progenitor cells in the neural folds are multipotent, having the ability to form multiple ectodermal derivatives, including epidermal, neural crest, and neural tube cells; (ii) the neural crest is an induced population that arises by interactions between the neural plate and the epidermis; and (iii) the competence of the neural plate to respond to inductive interactions changes as a function of embryonic age.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.pnas.org/content/93/18/9352.abstractPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC38431/PubMed CentralArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1996 National Academy of Sciences. This paper was presented at a colloquium entitled "Biology of Developmental Transcription Control, " organized by Eric H. Davidson, Roy J. Britten, and Gary Felsenfeld, held October 26-28, 1995, at the National Academy of Sciences in Irvine, CA. We are grateful to Drs. Kristin Artinger, Clare Baker, Roberto Mayor, and Catherine Krull for helpful comments on the manuscript. M.A.J.S. is currently supported by a Markey research fellowship. This work was partially supported by U.S. Public Health Service Grants HD25138 and NS34617 to M.B.-F. and by a grant from the March of Dimes Birth Defects Foundation. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
Lucille P. Markey Charitable TrustUNSPECIFIED
NIHHD-25138
U.S. Public Health Service (USPHS)NS34617
March of Dimes Birth Defects FoundationUNSPECIFIED
Subject Keywords:chicken embryo, neural development, peripheral nervous system, inductive interactions
Issue or Number:18
PubMed Central ID:PMC38431
Record Number:CaltechAUTHORS:20160405-100819036
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160405-100819036
Official Citation:M A Selleck and M Bronner-Fraser The genesis of avian neural crest cells: a classic embryonic induction PNAS 1996 93 (18) 9352-9357
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65925
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:06 Apr 2016 21:51
Last Modified:03 Oct 2019 09:51

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