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Removing orientation-induced localization biases in single-molecule microscopy using a broadband metasurface mask

Backlund, Mikael P. and Arbabi, Amir and Petrov, Peter N. and Arbabi, Ehsan and Saurabh, Saumya and Faraon, Andrei and Moerner, W. E. (2016) Removing orientation-induced localization biases in single-molecule microscopy using a broadband metasurface mask. Nature Photonics, 10 (7). pp. 459-462. ISSN 1749-4885. PMCID PMC5001689. doi:10.1038/nphoton.2016.93.

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Nanoscale localization of single molecules is a crucial function in several advanced microscopy techniques, including single-molecule tracking and wide-field super-resolution imaging. Until now, a central consideration of such techniques is how to optimize the precision of molecular localization. However, as these methods continue to push towards the nanometre size scale, an increasingly important concern is the localization accuracy. In particular, single fluorescent molecules emit with an anisotropic radiation pattern of an oscillating electric dipole, which can cause significant localization biases using common estimators. Here we present the theory and experimental demonstration of a solution to this problem based on azimuthal filtering in the Fourier plane of the microscope. We do so using a high-efficiency dielectric metasurface polarization/phase device composed of nanoposts with subwavelength spacing. The method is demonstrated both on fluorophores embedded in a polymer matrix and in dL5 protein complexes that bind malachite green.

Item Type:Article
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URLURL TypeDescription ReadCube access Information CentralArticle
Arbabi, Amir0000-0001-8831-7552
Arbabi, Ehsan0000-0002-5328-3863
Saurabh, Saumya0000-0002-7524-7548
Faraon, Andrei0000-0002-8141-391X
Moerner, W. E.0000-0002-2830-209X
Additional Information:© 2016 Macmillan Publishers Limited. Received 16 November 2015; accepted 11 April 2016; published online 16 May 2016. M.P.B. acknowledges support from the Robert and Marvel Kirby Stanford Graduate Fellowship. We also acknowledge support from National Institute of General Medical Sciences Grant No. 2R01GM085437 (to W.E.M.) and a National Science Foundation award (1512266) and the Defense Advanced Research Projects Agency (to A.F.). A.A. and E.A. were also supported by Samsung Electronics. We thank M. Bruchez for providing the plasmid for expressing recombinant dL5 protein and the MG ester fluorogen. Author Contributions: M.P.B. and W.E.M. conceived and designed the experiments. M.P.B. did the simulations and CRLB calculations. A.A., E.A. and A.F. designed, fabricated and characterized the mask. M.P.B. and P.N.P. performed experiments and analysed the data. S.S. prepared the dL5 samples. All the authors contributed to writing the paper. The authors declare no competing financial interests.
Group:Institute for Quantum Information and Matter
Funding AgencyGrant Number
Stanford UniversityUNSPECIFIED
Defense Advanced Research Projects Agency (DARPA)UNSPECIFIED
Samsung ElectronicsUNSPECIFIED
Issue or Number:7
PubMed Central ID:PMC5001689
Record Number:CaltechAUTHORS:20160405-131617866
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Official Citation:Removing orientation-induced localization biases in single-molecule microscopy using a broadband metasurface mask Mikael P. Backlund, Amir Arbabi, Petar N. Petrov, Ehsan Arbabi, Saumya Saurabh, Andrei Faraon & W. E. Moerner Nature Photonics (2016) doi:10.1038/nphoton.2016.93
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65936
Deposited By: George Porter
Deposited On:18 May 2016 20:34
Last Modified:27 Apr 2022 22:56

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