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A Distinct Intron-DNA Structure in Simian Virus 40 T-Antigen and Adenovirus 2 E1A Genes

Lee, Inho and Barton, Jacqueline K. (1993) A Distinct Intron-DNA Structure in Simian Virus 40 T-Antigen and Adenovirus 2 E1A Genes. Biochemistry, 32 (24). pp. 6121-6127. ISSN 0006-2960. doi:10.1021/bi00075a001.

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Distinct structures delineating the introns of simian virus 40 T-antigen and adenovirus 2 E1A genes have been discovered. The structures, which are centered around the branch points of the genes inserted in supercoiled double-stranded plasmids, are specifically targeted through photoactivated strand cleavage by the metal complex tris(4,7-diphenyl-1,10-phenanthroline)rhodium(III). The DNA sites that are recognized lack sequence homology but are similar in demarcating functionally important sites on the RNA level. The single-stranded DNA fragment corresponding to the coding strand of the E1A gene was also found to fold into a structure apparently identical to that in the supercoiled E1A gene based on the recognition by the metal complex. Further investigation of different single-stranded DNA fragments showed that the structure requires the sequences at both ends of the intron plus the flanking sequences but not the middle of the intron. These results provide direct evidence that the positions of these introns are structurally encoded on the DNA level.

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Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 1993 American Chemical Society. Received March 5, 1993; Revised Manuscript Received April 15, 1993. Publication Date: June 1993. We thank Prof. James L. Manley of Columbia University for the construction of the plasmids as well as for stimulating discussions at the outset of this project.
Issue or Number:24
Record Number:CaltechAUTHORS:20160405-140026351
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Official Citation:A distinct intron-DNA structure in simian virus 40 T-antigen and adenovirus 2 E1A genes Inho Lee and Jacqueline K. Barton Biochemistry 1993 32 (24), 6121-6127 DOI: 10.1021/bi00075a001
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65940
Deposited By: Ruth Sustaita
Deposited On:06 Apr 2016 18:23
Last Modified:10 Nov 2021 23:51

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