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Association of the Tetraspan Protein CD9 with Integrins on the Surface of S-16 Schwann Cells

Hadjiargyrou, Michael and Kaprielian, Zaven and Kato, Nobuo and Patterson, Paul H. (1996) Association of the Tetraspan Protein CD9 with Integrins on the Surface of S-16 Schwann Cells. Journal of Neurochemistry, 67 (6). pp. 2505-2513. ISSN 00223042. doi:10.1046/j.1471-4159.1996.67062505.x. https://resolver.caltech.edu/CaltechAUTHORS:20160407-073653521

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Abstract

The cell surface glycoprotein CD9 is a member of a group of proteins known as the “tetraspan” or “transmembrane 4 superfamily.” Previous work with non-neural cells has shown that CD9 associates in cis with integrins and small GTP-binding proteins on the cell surface. To extend our recent findings showing that perturbation of CD9 alters Schwann cell adhesion, proliferation, and migration, as well as neurite outgrowth in sympathetic neurons, we have searched for CD9-associated proteins in S-16 Schwann cells. We demonstrate here that CD9 is specifically coprecipitated from S-16 cell extracts by antibodies against integrins α3, α6, and β1. In addition, double immunofluorescence labeling and co-capping experiments indicate that CD9 is specifically colocalized with these integrins on the cell membrane of S-16 Schwann cells.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1046/j.1471-4159.1996.67062505.xDOIArticle
http://onlinelibrary.wiley.com/doi/10.1046/j.1471-4159.1996.67062505.x/abstractPublisherArticle
Additional Information:© 1996 International Society for Neurochemistry. Resubmitted manuscript received July 11, 1996; accepted July 22, 1996. Article first published online: 23 Nov 2002. We thank Kristine Venstrom and Louis Reichardt for providing the integrin antibodies, Doreen McDowell for media preparation, and Flora DePablo, Lisa Banner, and Reto Gadient for constructive comments on the manuscript. This work was supported by an NINDS grant to P.H.P. and an NIH Training grant fellowship to M.H.
Funders:
Funding AgencyGrant Number
National Institute of Neurological Disorders and Stroke (NINDS)UNSPECIFIED
NIH Training GrantUNSPECIFIED
Subject Keywords:Target of an antiproliferative antibody TAPA-1; CD9 protein; Schwann cells; Integrins; Tetraspan proteins
Issue or Number:6
DOI:10.1046/j.1471-4159.1996.67062505.x
Record Number:CaltechAUTHORS:20160407-073653521
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160407-073653521
Official Citation:Hadjiargyrou, M., Kaprielian, Z., Kato, N. and Patterson, P. H. (1996), Association of the Tetraspan Protein CD9 with Integrins on the Surface of S-16 Schwann Cells. Journal of Neurochemistry, 67: 2505–2513. doi: 10.1046/j.1471-4159.1996.67062505.x
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65977
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:07 Apr 2016 15:35
Last Modified:10 Nov 2021 23:51

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