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Rh(Phen)_2Phi^(3+) as a Shape-Selective Probe of Triple Helices

Lim, Ai Ching and Barton, Jacqueline K. (1998) Rh(Phen)_2Phi^(3+) as a Shape-Selective Probe of Triple Helices. Biochemistry, 37 (25). pp. 9138-9146. ISSN 0006-2960. https://resolver.caltech.edu/CaltechAUTHORS:20160407-101623917

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Abstract

RNA pur•pur-pyr and pyr•pur-pyr (pur = purine, pyr = pyrimidine) triple helices consisting of a Watson−Crick base-paired 28mer hairpin duplex and a Hoogsteen base-paired purine or pyrimidine 12mer are targeted with photoactivated cleavage by the metal complex Rh(phen)_2phi^(3+) (phen = phenanthroline, phi = 9,10-phenanthrenequinone diimine). The metal complex interacts with these triple helices in a structure-specific manner. Different cleavage patterns are seen with the pyr•pur-pyr and pur•pur-pyr motifs. Cleavage is seen on both of the Watson−Crick strands of the former motif and primarily on the purine Watson−Crick strand of the latter motif. Little cleavage is seen on the Hoogsteen strand for either motif. Importantly, the metal complex shows no detectable cleavage on the A-form RNA duplex in the absence of the third Hoogsteen strand. The cleavage patterns are consistent with an intercalated model for the metal complex in the triple helix. Similar cleavage is seen on DNA triple helices, but over a background of duplex cleavage. Targeting of synthetic RNA triple helices, but not duplex regions, by Rh(phen)_2phi^(3+) provides a basis for the chemical probing of triply bonded sites in folded RNA molecules.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/bi980509vDOIArticle
http://pubs.acs.org/doi/abs/10.1021/bi980509vPublisherArticle
ORCID:
AuthorORCID
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 1998 American Chemical Society. Received March 4, 1998; Revised Manuscript Received April 29, 1998. Publication Date (Web): June 5, 1998. We thank E. Theil for helpful suggestions during the course of this research.
Issue or Number:25
Record Number:CaltechAUTHORS:20160407-101623917
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160407-101623917
Official Citation:Rh(Phen)2Phi3+ as a Shape-Selective Probe of Triple Helices Ai Ching Lim and Jacqueline K. Barton Biochemistry 1998 37 (25), 9138-9146 DOI: 10.1021/bi980509v
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65983
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:07 Apr 2016 18:06
Last Modified:03 Oct 2019 09:52

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