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A Versatile Mismatch Recognition Agent: Specific Cleavage of a Plasmid DNA at a Single Base Mispair

Jackson, Brian A. and Alekseyev, Viktor Y. and Barton, Jacqueline K. (1999) A Versatile Mismatch Recognition Agent: Specific Cleavage of a Plasmid DNA at a Single Base Mispair. Biochemistry, 38 (15). pp. 4655-4662. ISSN 0006-2960. doi:10.1021/bi990255t.

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[Rh(bpy)_2(chrysi)]^(3+) is a novel, sterically bulky DNA intercalator that has been designed to bind specifically in the destabilized regions near DNA base mismatches and, upon photoactivation, to cleave the DNA backbone. Here the molecule is shown to be both a general and remarkably specific mismatch recognition agent. Specific DNA cleavage is observed at over 80% of mismatch sites in all the possible single base pair sequence contexts around the mispaired bases. Moreover, the complex is highly site-specific; it is shown to recognize and photocleave at a single base mismatch in a 2725 base pair linearized plasmid heteroduplex. Sterically demanding intercalators such as [Rh(bpy)_2(chrysi)]^(3+) may have application both in mutation detection systems and as mismatch-specific chemotherapeutic agents.

Item Type:Article
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Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 1999 American Chemical Society. Received February 3, 1999; Revised Manuscript Received February 26, 1999. Publication Date (Web): March 23, 1999. Support from the National Institutes of Health (GM33309) is gratefully acknowledged. B.A.J. acknowledges the NSF and the Parsons Foundation for predoctoral fellowships, and V.Y.A. acknowledges the HHMI and the Caltech SURF program for sponsorship of a summer undergraduate research fellowship.
Funding AgencyGrant Number
Ralph M. Parsons FoundationUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Caltech Summer Undergraduate Research Fellowship (SURF)UNSPECIFIED
Issue or Number:15
Record Number:CaltechAUTHORS:20160407-115523781
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Official Citation:A Versatile Mismatch Recognition Agent:  Specific Cleavage of a Plasmid DNA at a Single Base Mispair Brian A. Jackson,Viktor Y. Alekseyev, and, and Jacqueline K. Barton Biochemistry 1999 38 (15), 4655-4662 DOI: 10.1021/bi990255t
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65991
Deposited By: Ruth Sustaita
Deposited On:11 Apr 2016 17:33
Last Modified:10 Nov 2021 23:52

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