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Relationship between spatially restricted Krox-20 gene expression in branchial neural crest and segmentation in the chick embryo hindbrain

Nieto, M. Angela and Sechrist, John and Wilkinson, David G. and Bronner-Fraser, Marianne (1995) Relationship between spatially restricted Krox-20 gene expression in branchial neural crest and segmentation in the chick embryo hindbrain. EMBO Journal, 14 (8). pp. 1697-1710. ISSN 0261-4189. PMCID PMC398263. https://resolver.caltech.edu/CaltechAUTHORS:20160407-115541043

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Abstract

Previous studies have suggested that the rostrocaudal patterning of branchial arches in the vertebrate embryo derives from a coordinate segmental specification of gene expression in rhombomeres (r) and neural crest. However, expression of the Krox-20 gene is restricted to neural crest cells migrating to the third branchial arch, apparently from r5, whereas this rhombomere contributes cells to both the second and third arches. We examined in the chick embryo how this spatially restricted expression is established. Expression occurs in precursors in both r5 and r6, and we show by cell labelling that both rhombomeres contribute to Krox-20-expressing neural crest, emigration occurring first from r6 and later caudally from r5. Krox-20 transcripts are not detected in some precursors in rostral r5, presaging the lack of expression in cells migrating rostrally from this rhombomere. After transposition of r6 to the position of r4 or r5, many Krox-20-expressing cells migrate rostral to the otic vesicle, whereas when r5 is transplanted to the position of r4, only a small number of migrating cells express Krox-20. These results indicate that, in the chick, Krox-20 expression in branchial neural crest does not correlate with rhombomeric segmentation, and that there may be intrinsic differences in regulation between the r5 and r6 Krox-20-expressing populations.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC398263/PubMed CentralArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1995 Oxford University Press. Communicated by R.Krumlauf. Received on October 11, 1994; revised on December 19, 1994. We thank Brad Martinsen for excellent technical assistance with fixation of embryos and photoconversion of the DiI, Simone Lutolf for help with the cryostat sections and some DiI injections, and Mark Selleck and Kristin Artinger for help with image processing. We are also grateful to Claudio Stern for improvements to the in situ hybridization protocol and advice regarding DiI photoconversion, and to Robb Krumlauf and Scott Fraser for discussions and critical reading of the manuscript. This work was supported by USPHS HD-25138 to M.B.-F., by the Medical Research Council (D.G.W.), and by a grant from the Spanish Ministry of Education (DGICYT-PB92-0045) to M.A.N.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)HD-25138
Medical Research Council (UK)UNSPECIFIED
Ministerio de Educación y Ciencia (MEC)DGICYT-PB92-0045
Subject Keywords:branchial arches; hindbrain segmentation; Hox code; Krox-20; neural crest
Issue or Number:8
PubMed Central ID:PMC398263
Record Number:CaltechAUTHORS:20160407-115541043
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160407-115541043
Official Citation:Nieto, M. A., Sechrist, J., Wilkinson, D. G., & Bronner-Fraser, M. (1995). Relationship between spatially restricted Krox-20 gene expression in branchial neural crest and segmentation in the chick embryo hindbrain. The EMBO Journal, 14(8), 1697–1710.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65994
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:13 Apr 2016 18:21
Last Modified:03 Oct 2019 09:52

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