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Molecular Dissection of the AGAMOUS Control Region Shows That cis Elements for Spatial Regulation Are Located Intragenically

Sieburth, Leslie E. and Meyerowitz, Elliot M. (1997) Molecular Dissection of the AGAMOUS Control Region Shows That cis Elements for Spatial Regulation Are Located Intragenically. Plant Cell, 9 (3). pp. 355-365. ISSN 1040-4651. PMCID PMC156923. https://resolver.caltech.edu/CaltechAUTHORS:20160411-150154902

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Abstract

AGAMOUS (AG) is an Arabidopsis MADS box gene required for the normal development of the internal two whorls of the flower. AG RNA accumulates in distinct patterns early and late in flower development, and several genes have been identified as regulators of AG gene expression based on altered AG RNA accumulation in mutants. To understand AG regulatory circuits, we are now identifying cis regulatory domains by characterizing AG::β-glucuronidase (GUS) gene fusions. These studies show that a normal AG::GUS staining pattern is conferred by a 9.8-kb region encompassing 6 kb of upstream sequences and 3.8 kb of intragenic sequences. Constructs lacking the 3.8-kb intragenic sequences confer a GUS staining pattern that deviates both spatially and temporally from normal AG expression. The GUS staining patterns in the mutants for the three negative regulators of AG, apetala2, leunig, and curly leaf, showed the predicted change of expression for the construct containing the intragenic sequences, but no significant change was observed for the constructs lacking this intragenic region. These results suggest that intragenic sequences are essential for AG regulation and that these intragenic sequences contain the ultimate target sites for at least some of the known regulatory molecules.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1105/tpc.9.3.355DOIArticle
http://www.plantcell.org/content/9/3/355.abstractPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC156923/PubMed CentralArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1997 American Society of Plant Biologists. Received November 1, 1996; accepted January 16, 1997. We thank Justin Goodrich for allowing us to use clf-2, Esther Koh for her assistance with plant transformations, Gary Drews for the plasmid pLAGE-13, Zhongchi Liu for use of lug-3, Michael Deyholos for assistance in sequence comparisons, and Mike Frohlich for assistance with the initial analysis of these floral mutant lines. This work was supported by a Damon Runyon-Walter Winchell Cancer Research Fellowship (Grant No. DRG1081 to L.E.S.) and by the National Institutes of Health (Grant No. GM45697 to E.M.M.).
Funders:
Funding AgencyGrant Number
Damon Runyon-Walter Winchell Cancer FundDRG1081
NIHGM45697
Issue or Number:3
PubMed Central ID:PMC156923
Record Number:CaltechAUTHORS:20160411-150154902
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160411-150154902
Official Citation:L E Sieburth and E M Meyerowitz Molecular dissection of the AGAMOUS control region shows that cis elements for spatial regulation are located intragenically. Plant Cell 1997 9: 355-65. doi:10.1105/tpc.9.3.355
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66057
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:11 Apr 2016 22:34
Last Modified:03 Oct 2019 09:53

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