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Segmentation of the vertebrate hindbrain: a time-lapse analysis

Kulesa, Paul M. and Fraser, Scott E. (1998) Segmentation of the vertebrate hindbrain: a time-lapse analysis. International Journal of Developmental Biology, 42 (3). pp. 385-392. ISSN 0214-6282. http://resolver.caltech.edu/CaltechAUTHORS:20160412-082009384

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Abstract

The chick hindbrain starts from a simple and relatively uniform axis and becomes segmented into repeating units, called rhombomeres. The rhombomeres become sites of cell differentiation into specific neurons and the location from which neural crest cells emerge from the neural tube to form the peripheral nervous system, which has only been analyzed at distinct time points due to the lack of a method to watch the neural tube as it is shaped into segments. We have developed a whole-embryo explant culture system in order to study cell and tissue movements with time-lapse video microscopy. Quantitative analyses of the neural tube during its segmentation show that not all rhombomeres are shaped by the same mechanism. In the rostral hindbrain, or first three segments, rhombomeres are shaped by an expansion in the lateral width of the mid-rhombomere; either a smaller expansion or a constriction takes place at the rhombomere boundaries. In the caudal hindbrain, the rhombomere boundaries constrict more than the mid-rhombomere lateral widths increase or decrease, leading to the shaping of the segments. Throughout the segmentation process the rostrocaudal lengths of all rhombomeres remain nearly constant indicating that shape changes are influenced by lateral expansions and constrictions of the neural tube.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.intjdevbiol.com/paper.php?doi=9654023PublisherArticle
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5739-4026
Additional Information:© 1998 UBC Press.
Subject Keywords:vertebrate, hindbrain, segmentation, whole-embryo explant, time-lapse
Record Number:CaltechAUTHORS:20160412-082009384
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160412-082009384
Official Citation:Segmentation of the vertebrate hindbrain: a time-lapse analysis. P M Kulesa and S E Fraser Int. J. Dev. Biol. (1998) 42: 385-392
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66069
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:12 Apr 2016 16:18
Last Modified:12 Apr 2016 16:18

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