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SpRunt-1, a New Member of the Runt Domain Family of Transcription Factors, Is a Positive Regulator of the Aboral Ectoderm-Specific CyIIIA Gene in Sea Urchin Embryos

Coffman, James A. and Kirchhamer, Carmen V. and Harrington, Michael G. and Davidson, Eric H. (1996) SpRunt-1, a New Member of the Runt Domain Family of Transcription Factors, Is a Positive Regulator of the Aboral Ectoderm-Specific CyIIIA Gene in Sea Urchin Embryos. Developmental Biology, 174 (1). pp. 43-54. ISSN 0012-1606. http://resolver.caltech.edu/CaltechAUTHORS:20160412-102830615

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Abstract

In this paper we present a structural and functional characterization of a new sea urchin embryo transcription factor, SpRunt-1. This factor was isolated by means of its specific interaction with acis-regulatory target site of theCyIIIagene. Here we show that this target site, the P7I site, is required for normal embryonic activation ofCyIIIa·CATreporter gene constructs. An oligonucleotide affinity column bearing the P7I target site purifies a 21-kDa polypeptide from blastula-stage nuclear extracts, and the amino acid sequence obtained from this polypeptide was used to generate a nucleic acid probe with which the corresponding cDNA was cloned. The cDNA encodes an approximately 60-kDa protein, SpRunt-1, which includes a “runt domain” that is closely homologous to those ofDrosophilaand mammalian runt domain transcription factors. RNA and genomic blots show that SpRunt-1 is represented by a single embryonic transcript, encoded by one of possibly two runt-domain-containing genes. By RNA probe protection we found that transcripts of SpRunt-1 increase in concentration dramatically after the blastula stage of development, suggesting that the up-regulation ofCyIIIathat occurs after blastula stage is a function of zygotically transcribed SpRunt-1. These results are discussed with reference to known features of the runt domain family of transcription factors.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/dbio.1996.0050DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160696900500PublisherArticle
ORCID:
AuthorORCID
Harrington, Michael G.0000-0002-7923-8032
Additional Information:© 1996 by Academic Press, Inc. The authors are indebted to Dr. Jun Xian, who constructed the pSPORT cDNA library from which SpRunt-1 was isolated. We thank Megan Andrews, who obtained some of the DNA sequences reported in this paper. We also thank Drs. Ellen Rothenberg and Carl Parker for critical reviews of this paper. This work was supported by NIH Grant HD-05753, by the Beckman Institute, by a subcontract from NSF Science and Technology Grant BIR9214821, and by ONR Grant N00014-93-1-0379.
Funders:
Funding AgencyGrant Number
NIHHD-05753
Caltech Beckman InstituteUNSPECIFIED
NSFBIR9214821
Office of Naval Research (ONR)N00014-93-1-0379
Record Number:CaltechAUTHORS:20160412-102830615
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160412-102830615
Official Citation:James A. Coffman, Carmen V. Kirchhamer, Michael G. Harrington, Eric H. Davidson, SpRunt-1, a New Member of the Runt Domain Family of Transcription Factors, Is a Positive Regulator of the Aboral Ectoderm-SpecificCyIIIA Gene in Sea Urchin Embryos, Developmental Biology, Volume 174, Issue 1, 25 February 1996, Pages 43-54, ISSN 0012-1606, http://dx.doi.org/10.1006/dbio.1996.0050. (http://www.sciencedirect.com/science/article/pii/S0012160696900500)
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ID Code:66083
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:12 Apr 2016 17:47
Last Modified:12 Apr 2016 17:48

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