Minor, Joseph E. and Lee, James J. and Akhurst, Rosemary J. and Leahy, Patrick S. and Britten, Roy J. and Davidson, Eric H. (1987) Sea Urchin Actin Gene Linkages Determined by Genetic Segregation. Developmental Biology, 122 (1). pp. 291-295. ISSN 0012-1606. doi:10.1016/0012-1606(87)90354-X. https://resolver.caltech.edu/CaltechAUTHORS:20160412-142332974
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Abstract
Genetic linkage between the actin genes of Strongylocentrotus purpuratus was investigated by observing the segregation of restriction fragment length polymorphisms (RFLPs). Specific RFLPs of actin gene pairs CyICyIIa and CyIIIaCyIIIb always cosegregated, confirming the linkage groups CyI-CyIIa-CyIIb and CyIIIa-CyIIIb previously determined by molecular cloning. In contrast, RFLPs of actin genes CyICyIIa, CyIIIaCyIIIb, and M all segregated at random with respect to one another. This demonstrates that the known actin gene clusters CyI-CyIIa-CyIIb, CyIIIa-CyIIIb, and the M actin gene are not closely linked.
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| Additional Information: | © 1987 Academic Press, Inc. Received 2 January 1987, Accepted 6 March 1987. We thank Dr. Elliot Meyerowitz for reviewing the manuscript. This research was supported by an NIH grant (GM-20927). J.E.M. was supported by an NSF predoctoral fellowship, J.J.L. by an NIH training grant (GM-07616), and R.J.A. by an EMBO fellowship. | |||||||||
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| Issue or Number: | 1 | |||||||||
| DOI: | 10.1016/0012-1606(87)90354-X | |||||||||
| Record Number: | CaltechAUTHORS:20160412-142332974 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160412-142332974 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 66089 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Ruth Sustaita | |||||||||
| Deposited On: | 12 Apr 2016 21:32 | |||||||||
| Last Modified: | 10 Nov 2021 23:53 |
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