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Translocation of Latex Beads after Laser Ablation of the Avian Neural Crest

Coulombe, James N. and Bronner-Fraser, Marianne (1984) Translocation of Latex Beads after Laser Ablation of the Avian Neural Crest. Developmental Biology, 106 (1). pp. 121-134. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160412-143132806

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Abstract

Previous studies from this laboratory (M. E. Bronner-Fraser, 1982, Dev. Biol. 91, 50–63) have demonstrated that latex beads translocate ventrally after injection into avian embryos during the phase of neural crest migration, to settle in the vicinity of neural-crest-derived structures. In order to examine the role of host neural crest cells in the ventral translocation of implanted beads, latex beads have been injected into regions of embryos from which the neural crest cells have been ablated using a laser microbeam. Prior to their migratory phase, neural crest cells reside in the dorsal portion of the neural tube. Laser irradiation of the dorsal neural tube was used to reproducibly achieve either partial or complete ablation of neural crest cells from the irradiated regions. The effectiveness of the ablation was assessed by the degree of reduction in dorsal root ganglia, a neural crest derivative. Because of the rapidity and precision of this technique, it was possible to selectively remove neural crest cells without significantly altering other embryonic structures. The results indicate that, after injection of latex beads into the somites of embryos whose neural crest cells were removed by laser irradiation, the beads translocate ventrally in the absence of the endogenous neural crest.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(84)90068-XDOIArticle
http://www.sciencedirect.com/science/article/pii/001216068490068XPublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1984 Academic Press, Inc. Received February 7, 1984; accepted in revised form June 15, 1984. We thank Dr. Scott Fraser for his careful reading of the manuscript, Georgia Guillory and Leena Carriere for their technical assistance, Virginia Bayer for illustrations, and Dirk van Dyke for design of the circuit linking the motorized stage to the laser Q switch. We are grateful to Dr. Michael Berns and the Laser Microbeam Program of the NIH (RR-01192-01) where these experiments were performed. This work was supported by US Public Health Service Grant HD-15527-01 and by Basil O'Connor Starter Research Grant 5-312 from the March of Dimes Birth Defects Foundation.
Funders:
Funding AgencyGrant Number
NIHRR-01192-01
U.S. Public Health Service (USPHS)HD-15527-01
March of Dimes Birth Defects Foundation 5-312
Issue or Number:1
Record Number:CaltechAUTHORS:20160412-143132806
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160412-143132806
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66090
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:12 Apr 2016 22:10
Last Modified:03 Oct 2019 09:53

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