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Distribution of Latex Beads and Retinal Pigment Epithelial Cells along the Ventral Neural Crest Pathway

Bronner-Fraser, Marianne (1982) Distribution of Latex Beads and Retinal Pigment Epithelial Cells along the Ventral Neural Crest Pathway. Developmental Biology, 91 (1). pp. 50-63. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160413-111515282

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Abstract

Neural crest cells migrate along defined pathways in the trunk of avian embryos. Previous studies have demonstrated that crest-derived pigment cells migrated ventrally after injection onto the ventral neural crest pathway (M. E. Bronner-Fraser and A. M. Cohen, 1980, Develop. Biol. 77, 130–141). In the present study, latex polystyrene beads and retinal pigment epithelial (RPE) cells were injected onto the ventral pathway in order to probe the environment along this migratory route. Although the RPE cells are nonmotile and not derived from the neural crest, they also translocated ventrally. Thus, factors independent of active migration may affect the localization of RPE cells (and endogenous crest cells). To test this possibility, latex polystyrene beads were injected onto the ventral pathway. Three types of beads were used: (a) uncoated latex beads; (b) latex beads coated with bovine serum albumin (BSA-beads); and (c) latex beads coated with fibronectin (FN-beads). Uncoated and BSA-beads distributed along the ventral pathway similarly to RPE cells and endogenous crest cells. However, FN-beads remained near the site of implantation and did not move ventrally. The results suggest (1) that molecules like fibronectin on the cell surface might serve as a recognition mechanism that prevents entrance onto the ventral pathway; and (2) that crest cell localization may, in part, be influenced by a driving force imparted by the embryonic environment.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(82)90007-0DOIArticle
http://www.sciencedirect.com/science/article/pii/0012160682900070PublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1982 Academic Press, Inc. Received October 19, 1981; accepted in revised form December 11, 1981. I would like to thank Drs. S. E. Fraser and A. Warner for helpful suggestions regarding the manuscript, and Leena Carriere for her technical assistance. This research was supported by U. S. Public Health Service Grant HD-15527-01 and by Basil O'Connor Strater Research Grant 5-312 from the March of Dimes Birth Defects Foundation awarded to M. Bronner-Fraser.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)HD-15527-01
March of Dimes Birth Defects Foundation5-312
Issue or Number:1
Record Number:CaltechAUTHORS:20160413-111515282
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160413-111515282
Official Citation:Marianne Bronner-Fraser, Distribution of latex beads and retinal pigment epithelial cells along the ventral neural crest pathway, Developmental Biology, Volume 91, Issue 1, 1982, Pages 50-63, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(82)90007-0. (http://www.sciencedirect.com/science/article/pii/0012160682900070)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66116
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:13 Apr 2016 18:36
Last Modified:03 Oct 2019 09:53

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