Distribution of Latex Beads and Retinal Pigment Epithelial Cells along the Ventral Neural Crest Pathway

Abstract

Neural crest cells migrate along defined pathways in the trunk of avian embryos. Previous studies have demonstrated that crest-derived pigment cells migrated ventrally after injection onto the ventral neural crest pathway (M. E. Bronner-Fraser and A. M. Cohen, 1980, Develop. Biol. 77, 130–141). In the present study, latex polystyrene beads and retinal pigment epithelial (RPE) cells were injected onto the ventral pathway in order to probe the environment along this migratory route. Although the RPE cells are nonmotile and not derived from the neural crest, they also translocated ventrally. Thus, factors independent of active migration may affect the localization of RPE cells (and endogenous crest cells). To test this possibility, latex polystyrene beads were injected onto the ventral pathway. Three types of beads were used: (a) uncoated latex beads; (b) latex beads coated with bovine serum albumin (BSA-beads); and (c) latex beads coated with fibronectin (FN-beads). Uncoated and BSA-beads distributed along the ventral pathway similarly to RPE cells and endogenous crest cells. However, FN-beads remained near the site of implantation and did not move ventrally. The results suggest (1) that molecules like fibronectin on the cell surface might serve as a recognition mechanism that prevents entrance onto the ventral pathway; and (2) that crest cell localization may, in part, be influenced by a driving force imparted by the embryonic environment.