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Developmental Patterns of Cytoplasmic Transcript Prevalence in Sea Urchin Embryos

Flytzanis, Constantin N. and Brandhorst, Bruce P. and Britten, Roy J. and Davidson, Eric H. (1982) Developmental Patterns of Cytoplasmic Transcript Prevalence in Sea Urchin Embryos. Developmental Biology, 91 (1). pp. 27-35. ISSN 0012-1606. http://resolver.caltech.edu/CaltechAUTHORS:20160413-111515543

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Abstract

Several hundred two-cell stage, gastrula stage, and pluteus stage sea urchin embryo cDNA clones were screened with [^(32)P]cDNA transcribed from a developmental series of cytoplasmic and polysomal poly(A) RNAs. Transcript prevalence was estimated by reference to the reaction of a series of standard clones complementary to RNAs of known abundance. We describe the dominant pattern of prevalence change during development for abundant sequences, i.e., those present in the range 5 × 10^4–10^6 molecules/embryo. These transcripts are most often about equally abundant in egg and pluteus stage embryos, and are severalfold less prevalent at gastrula stage. Less than 10% of the sequences displaying this pattern undergo >10-fold changes in prevalence during development. A search for later embryo sequences not represented detectably in egg or cleavage stage embryo poly(A) RNA yielded a number of examples. These were confirmed by the RNA gel blot method. Sequences regulated in this manner all belonged to lower abundance classes, and are present at about 40 copies/cell or less at the gastrula stage.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(82)90004-5DOIArticle
http://www.sciencedirect.com/science/article/pii/0012160682900045PublisherArticle
Additional Information:© 1982 Academic Press, Inc. Received September 8, 1981; accepted in revised form December 4, 1981. This research was supported by NIH Grant HD-05753. C.N.F. was supported by a fellowship from the Deutsches Krebsforschungszentrum, Heidelberg, West Germany. B.P.B. was aided by a travel grant from the National Sciences and Engineering Research Council of Canada.
Funders:
Funding AgencyGrant Number
NIHHD-05753
Deutsches KrebsforschungszentrumUNSPECIFIED
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
Record Number:CaltechAUTHORS:20160413-111515543
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160413-111515543
Official Citation:Constantin N. Flytzanis, Bruce P. Brandhorst, Roy J. Britten, Eric H. Davidson, Developmental patterns of cytoplasmic transcript prevalence in sea urchin embryos, Developmental Biology, Volume 91, Issue 1, 1982, Pages 27-35, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(82)90004-5. (http://www.sciencedirect.com/science/article/pii/0012160682900045)
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ID Code:66117
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:13 Apr 2016 19:55
Last Modified:13 Apr 2016 19:55

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