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Developmental Patterns of Cytoplasmic Transcript Prevalence in Sea Urchin Embryos

Flytzanis, Constantin N. and Brandhorst, Bruce P. and Britten, Roy J. and Davidson, Eric H. (1982) Developmental Patterns of Cytoplasmic Transcript Prevalence in Sea Urchin Embryos. Developmental Biology, 91 (1). pp. 27-35. ISSN 0012-1606. doi:10.1016/0012-1606(82)90004-5.

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Several hundred two-cell stage, gastrula stage, and pluteus stage sea urchin embryo cDNA clones were screened with [^(32)P]cDNA transcribed from a developmental series of cytoplasmic and polysomal poly(A) RNAs. Transcript prevalence was estimated by reference to the reaction of a series of standard clones complementary to RNAs of known abundance. We describe the dominant pattern of prevalence change during development for abundant sequences, i.e., those present in the range 5 × 10^4–10^6 molecules/embryo. These transcripts are most often about equally abundant in egg and pluteus stage embryos, and are severalfold less prevalent at gastrula stage. Less than 10% of the sequences displaying this pattern undergo >10-fold changes in prevalence during development. A search for later embryo sequences not represented detectably in egg or cleavage stage embryo poly(A) RNA yielded a number of examples. These were confirmed by the RNA gel blot method. Sequences regulated in this manner all belonged to lower abundance classes, and are present at about 40 copies/cell or less at the gastrula stage.

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Additional Information:© 1982 Academic Press, Inc. Received September 8, 1981; accepted in revised form December 4, 1981. This research was supported by NIH Grant HD-05753. C.N.F. was supported by a fellowship from the Deutsches Krebsforschungszentrum, Heidelberg, West Germany. B.P.B. was aided by a travel grant from the National Sciences and Engineering Research Council of Canada.
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Deutsches KrebsforschungszentrumUNSPECIFIED
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
Issue or Number:1
Record Number:CaltechAUTHORS:20160413-111515543
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Official Citation:Constantin N. Flytzanis, Bruce P. Brandhorst, Roy J. Britten, Eric H. Davidson, Developmental patterns of cytoplasmic transcript prevalence in sea urchin embryos, Developmental Biology, Volume 91, Issue 1, 1982, Pages 27-35, ISSN 0012-1606, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66117
Deposited By: Ruth Sustaita
Deposited On:13 Apr 2016 19:55
Last Modified:10 Nov 2021 23:53

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