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Proteins of the Sea Urchin Egg Vitelline Layer

Niman, H. L. and Hough-Evans, B. R. and Vacquier, V. D. and Britten, R. J. and Lerner, R. A. and Davidson, E. H. (1984) Proteins of the Sea Urchin Egg Vitelline Layer. Developmental Biology, 102 (2). pp. 390-401. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160414-070442729

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Abstract

The vitelline layers (VL) of unfertilized sea urchin eggs were isolated, and the diversity of their polypeptide constitutents estimated by two-dimensional polyacrylamide gel electrophoresis. At least 25 components are reproducibly observed. While VL polypeptides are almost certainly synthesized in the growing oocyte, they are not among the more prevalent newly synthesized proteins detected in oocytes that were isolated and labeled in vitro for 4 hr. A set of monoclonal antibodies was raised against VL components and partially characterized. The 31 monoclonals analyzed fell into 11 classes with respect to their avidity for VL proteins solubilized under mild and under strongly denaturing conditions, and to their reactions with surface components of the VLs of living eggs. Fluorescence microscopy showed diverse patterns of surface reactivity when different monoclonal antibodies were compared. Two of the monoclonal antibodies reacted with specific sets of three proteins each on VL protein blots. It is concluded that the VL is a complex structure containing a large number of different polypeptide components, the genes for several of which should now be experimentally accessible.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(84)90203-3DOIArticle
http://www.sciencedirect.com/science/article/pii/0012160684902033PublisherArticle
Additional Information:© 1984 Academic Press Inc. Received June 30, 1983; accepted in revised form November 18, 1983. We thank D. Schloeder for help with the isolation and maintenance of the hybridomas, and C. O'Connor for instruction in two-dimensional gel electrophoresis. The assistance of Patrick Leahy in the preparation of the VLs used for this study is gratefully acknowledged. E. J. Carroll, Jr. and E. Rothenberg provided helpful criticisms of the manuscript. This research was supported by NIH Grants BRSG-RR-07003, HD-05753 (to E.H.D.), CA-25803 (to R.A.L.), and HD-12986 (to V.D.V). This is manuscript number 3105 IMM from the Research Institute of Scripps Clinic.
Funders:
Funding AgencyGrant Number
NIHBRSG-RR-07003
NIHHD-05753
NIHCA-25803
NIHHD-12986
Other Numbering System:
Other Numbering System NameOther Numbering System ID
Research Institute of Scripps Clinic Manuscript. no. 3105
Issue or Number:2
Record Number:CaltechAUTHORS:20160414-070442729
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160414-070442729
Official Citation:H.L. Niman, B.R. Hough-Evans, V.D. Vacquier, R.J. Britten, R.A. Lerner, E.H. Davidson, Proteins of the sea urchin egg vitelline layer, Developmental Biology, Volume 102, Issue 2, 1984, Pages 390-401, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(84)90203-3. (http://www.sciencedirect.com/science/article/pii/0012160684902033)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66138
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Apr 2016 17:12
Last Modified:03 Oct 2019 09:53

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