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Accumulation of Individual Histone mRNAs during Embryogenesis of the Sea Urchin Strongylocentrotus purpuratus

Mauron, Alex and Kedes, Larry and Hough-Evans, Barbara R. and Davidson, Eric H. (1982) Accumulation of Individual Histone mRNAs during Embryogenesis of the Sea Urchin Strongylocentrotus purpuratus. Developmental Biology, 94 (2). pp. 425-434. ISSN 0012-1606. http://resolver.caltech.edu/CaltechAUTHORS:20160414-070445765

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Abstract

We have determined that the stockpile of maternal histone mRNAs for both H2B and H3 in eggs of the sea urchin Strongylocentrotus purpuratus corresponds to 0.8–1 million molecules per egg. This value is a somewhat smaller quantity than had previously been estimated. On the other hand, the increase brought about by embryonic mRNA synthesis is 10- to 12-fold, rather than 4-fold. Further analysis of the time course of accumulation of these mRNAs suggests that histone gene expression in early development involves a complex interaction between activation of stockpiled maternal messengers, fluctuating rates of histone gene transcription, and alterations of histone mRNA stability. The maternal pool of H1 mRNA is considerably smaller (80,000 molecules). During cleavage, it partially catches up and peaks at about half the value for H2B and H3. We also examined previtellogenic oocytes for the presence of histone mRNAs by the same methods and were unable to detect any. Thus the stockpile of maternal histone mRNAs must accumulate after the previtellogenic stage of oogenesis.


Item Type:Article
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http://dx.doi.org/10.1016/0012-1606(82)90359-1DOIArticle
http://www.sciencedirect.com/science/article/pii/0012160682903591PublisherArticle
Additional Information:© 1982 Academic Press, Inc. Received January 21, 1982; accepted in revised form May 7, 1982. This work was supported in part by grants from the National Institutes of Health to E.H.D. and to L.K. and the Veterans Administration (L.K.). A.M. was a fellow of the Swiss National Research Fund. L.K. is an Investigator of the Howard Hughes Medical Institute.
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NIHUNSPECIFIED
Veterans Administration UNSPECIFIED
Swiss National Research FundUNSPECIFIED
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Record Number:CaltechAUTHORS:20160414-070445765
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160414-070445765
Official Citation:Alex Mauron, Larry Kedes, Barbara R. Hough-Evans, Eric H. Davidson, Accumulation of individual histone mRNAs during embryogenesis of the sea urchin Strongylocentrotus purpuratus, Developmental Biology, Volume 94, Issue 2, 1982, Pages 425-434, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(82)90359-1. (http://www.sciencedirect.com/science/article/pii/0012160682903591)
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ID Code:66141
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Apr 2016 17:14
Last Modified:14 Apr 2016 17:14

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