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Cloned Embryo mRNAs Not Detectably Expressed in Adult Sea Urchin Coelomocytes

Xin, Ji-Hou and Brandhorst, Bruce P. and Britten, Roy J. and Davidson, Eric H. (1982) Cloned Embryo mRNAs Not Detectably Expressed in Adult Sea Urchin Coelomocytes. Developmental Biology, 89 (2). pp. 527-531. ISSN 0012-1606. http://resolver.caltech.edu/CaltechAUTHORS:20160414-073658938

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Abstract

A set of 455 cloned pluteus cDNAs was analyzed by colony hybridization to radioactive cDNAs transcribed from cytoplasmic poly(A) RNA of pluteus embryos and adult coelomocytes. The prevalence of transcripts corresponding to each of the clones was estimated by use of cloned standards having known transcript prevalences. While 46% of the clones correspond to transcripts present at more than 105 copies/ng poly(A) RNA (about 5 copies/cell) in plutei, only 5.5% are that prevalent in the coelomocyte. The most prevalent pluteus transcripts are frequently also prevalent in coelomocytes, but more than 85% of the moderately prevalent pluteus transcripts are below the limit of detection of about 1 copy/cell in coelomocytes. None of the clones corresponding to rare transcripts in the pluteus is represented by prevalent transcripts in coelomocytes. The data suggest that the expression of the majority of genes giving rise to moderately prevalent messenger RNAs in embryos must be regulated during the life cycle of the sea urchin, while some very prevalent transcripts may be ubiquitous.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(82)90342-6DOIArticle
http://www.sciencedirect.com/science/article/pii/0012160682903426PublisherArticle
Additional Information:© 1982 Academic Press, Inc. Received June 1, 1981; accepted in revised form August 27, 1981. This research was supported by NIH Grant HD-05753. J.-h.X. was supported by the Shanghai Institute of Biochemistry.
Funders:
Funding AgencyGrant Number
NIHHD-05753
Shanghai Institute of BiochemistryUNSPECIFIED
Record Number:CaltechAUTHORS:20160414-073658938
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160414-073658938
Official Citation:Ji-hou Xin, Bruce P. Brandhorst, Roy J. Britten, Eric H. Davidson, Cloned embryo mRNAs not detectably expressed in adult sea urchin coelomocytes, Developmental Biology, Volume 89, Issue 2, 1982, Pages 527-531, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(82)90342-6. (http://www.sciencedirect.com/science/article/pii/0012160682903426)
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ID Code:66143
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Apr 2016 17:53
Last Modified:14 Apr 2016 17:53

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