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Pattern Regulation in the Eyebud of Xenopus Studied with a Vital-Dye Fiber-Tracing Technique

O'Rourke, Nancy A. and Fraser, Scott E. (1986) Pattern Regulation in the Eyebud of Xenopus Studied with a Vital-Dye Fiber-Tracing Technique. Developmental Biology, 114 (2). pp. 277-288. ISSN 0012-1606. http://resolver.caltech.edu/CaltechAUTHORS:20160414-080122015

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Abstract

Evidence for pattern regulation in the developing Xenopus visual system has previously been obtained after surgical manipulations of the eyebud early in development. In one experimental paradigm, a “compound” eye is produced by combining a nasal (anterior) half-eyebud with normal dorsoventral polarity and a temporal (posterior) half-eyebud with inverted dorsoventral polarity. The adult retinotectal projection from such compound eyes, as assayed by electrophysiological mapping techniques, shows normal dorsoventral polarity in both halves, indicating an apparent reversal in the polarity of the surgically-inverted half. We have utilized a fluorescent vital-dye fiber-tracing technique to investigate the early events in this regulatory process. The results show that the change in dorsoventral polarity is not due to cell movements in the eyebud after surgery. Interestingly, the experiments also demonstrate that the pattern of connections initially formed by the developing eye does not reflect the pattern regulation observed in the adult retinotectal map; instead, the temporal half of the eye projects to the tectum with inverted dorsoventral order. Thus, the regulation observed in the adult does not become evident in the pattern of the projection until after early larval development.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(86)90192-2DOIArticle
http://www.sciencedirect.com/science/article/pii/0012160686901922PublisherArticle
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5739-4026
Additional Information:© 1986 Academic Press, Inc. Received March 1, 1985; accepted in revised form November 10, 1985. We thank R. Gimlich and J. Braun for their generous gift of some of the fluorescent dextrans used in this study; V. Bayer for her help with electrophysiological mapping; D. Carhart and D. Valentino for help with the histology; and M. Bronner-Fraser, J. Coulombe, D. Krotoski, and D. Valentino for their careful reading of the manuscript. Preliminary reports of some of this work have appeared (O'Rourke and Fraser, 1984, 1985). This work was supported by grants from the NSF (BNS80-23638, BNS84-06307, and BNS85-11224) and by a McKnight Foundation Scholar's Award.
Funders:
Funding AgencyGrant Number
NSFBNS80-23638
NSFBNS84-06307
NSFBNS85-11224
McKnight FoundationUNSPECIFIED
Record Number:CaltechAUTHORS:20160414-080122015
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160414-080122015
Official Citation:Nancy A. O'Rourke, Scott E. Fraser, Pattern regulation in the eyebud of Xenopus studied with a vital-dye fiber-tracing technique, Developmental Biology, Volume 114, Issue 2, 1986, Pages 277-288, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(86)90192-2. (http://www.sciencedirect.com/science/article/pii/0012160686901922)
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ID Code:66144
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Apr 2016 17:36
Last Modified:14 Apr 2016 17:36

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