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Introduction of Cloned DNA into Sea Urchin Egg Cytoplasm: Replication and Persistence during Embryogenesis

McMahon, Andrew P. and Flytzanis, Constantin N. and Hough-Evans, Barbara R. and Katula, Karen S. and Britten, Roy J. and Davidson, Eric H. (1985) Introduction of Cloned DNA into Sea Urchin Egg Cytoplasm: Replication and Persistence during Embryogenesis. Developmental Biology, 108 (2). pp. 420-430. ISSN 0012-1606. doi:10.1016/0012-1606(85)90045-4.

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Cloned DNA sequences were introduced into the cytoplasm of unfertilized sea urchin eggs by a simple microinjection technique. Sperm was then added, and development allowed to proceed. If linearized plasmids are injected they form random concatenates, and during the early development of the embryos replicate repeatedly. Eukaryotic sequences are not required for replication of the exogenous DNA. Injected supercoiled DNAs neither ligate nor replicate. Both forms of exogenous DNA persist in the embryo through pluteus stage.

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Additional Information:© 1985 Academic Press, Inc. Received September 7, 1984; accepted in revised farm November 7, 1984. We thank Frances Teng for expert and intelligent technical assistance. We are grateful to Anita Colin for advice on microinjection procedures, and to Professors Norman Davidson, Barbara Wold, and Ellen Rothenberg for helpful and critical reviews of the manuscript. This research was supported by NIH Grant HD-05753. A.P.M. and C.N.F. were supported by American Cancer Society, California Division fellowships (J-33-82 and S-11-83, respectively) and K.S.K. by an NIH postdoctoral training grant (HD-07257).
Funding AgencyGrant Number
American Cancer Society, California DivisionJ-33-82
American Cancer Society, California DivisionS-11-83
NIH Postdoctoral FellowshipHD-07257
Issue or Number:2
Record Number:CaltechAUTHORS:20160414-080122940
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Official Citation:Andrew P. McMahon, Constantin N. Flytzanis, Barbara R. Hough-Evans, Karen S. Katula, Roy J. Britten, Eric H. Davidson, Introduction of cloned DNA into sea urchin egg cytoplasm: Replication and persistence during embryogenesis, Developmental Biology, Volume 108, Issue 2, 1985, Pages 420-430, ISSN 0012-1606, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66145
Deposited By: Ruth Sustaita
Deposited On:14 Apr 2016 17:42
Last Modified:10 Nov 2021 23:53

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