Flytzanis, Constantin N. and McMahon, Andrew P. and Hough-Evans, Barbara R. and Katula, Karen S. and Britten, Roy J. and Davidson, Eric H. (1985) Persistence and Integration of Cloned DNA in Postembryonic Sea Urchins. Developmental Biology, 108 (2). pp. 431-442. ISSN 0012-1606. http://resolver.caltech.edu/CaltechAUTHORS:20160414-080123212
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Abstract
Cloned DNA was injected into the cytoplasm of unfertilized sea urchin eggs which were then fertilized and cultured in the laboratory through metamorphosis. The exogenous DNA replicated manyfold and persisted for weeks in a majority of growing larvae, as shown by hydridizing “dot blots” of the DNA of single individuals with appropriate labeled probes. After metamorphosis 5–15% of the juvenile sea urchins retained the exogenous sequences. Genomic integration of the exogenous sequence was observed in the DNA of a postmetamorphosis juvenile.
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| Additional Information: | © 1985 Academic Press, Inc. Received September 7, 1984; accepted in revised form November 7, 1984. It is our pleasure to acknowledge Mr. Edward Nolan for technical assistance, Mr. Patrick Leahy for culturing sea urchin embryos and larvae, and Dr. David Chapman for contributing cultures of Rhodomonas sp. Dr. Norman Davidson, Dr. Ellen Rothenberg, and Dr. Barbara Wold provided us with thoughtful comments on the manuscript, of which we are most appreciative. This research was supported by NIH Grant HD-05753. C.N.F. is a Lievre Fellow of the American Cancer Society, California Division, A.P.M. was supported by the same Society (Fellowship S-11-83 and J-33-82, respectively), and K.S.K. by an NIH postdoctoral training grant (HD-07257). | ||||||||||||
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| Record Number: | CaltechAUTHORS:20160414-080123212 | ||||||||||||
| Persistent URL: | http://resolver.caltech.edu/CaltechAUTHORS:20160414-080123212 | ||||||||||||
| Official Citation: | Constantin N. Flytzanis, Andrew P. McMahon, Barbara R. Hough-Evans, Karen S. Katula, Roy J. Britten, Eric H. Davidson, Persistence and integration of cloned DNA in postembryonic sea urchins, Developmental Biology, Volume 108, Issue 2, 1985, Pages 431-442, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(85)90046-6. (http://www.sciencedirect.com/science/article/pii/0012160685900466) | ||||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
| ID Code: | 66146 | ||||||||||||
| Collection: | CaltechAUTHORS | ||||||||||||
| Deposited By: | Ruth Sustaita | ||||||||||||
| Deposited On: | 14 Apr 2016 17:39 | ||||||||||||
| Last Modified: | 14 Apr 2016 17:39 |
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