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Effects of Different Fragments of the Fibronectin Molecule on Latex Bead Translocation along Neural Crest Migratory Pathways

Bronner-Fraser, Marianne (1985) Effects of Different Fragments of the Fibronectin Molecule on Latex Bead Translocation along Neural Crest Migratory Pathways. Developmental Biology, 108 (1). pp. 131-145. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160414-080345720

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Abstract

Previous studies from this laboratory have utilized latex beads as probes of embryonic migratory pathways. After microinjection into embryos at the time of neural crest migration, uncoated latex polystyrene beads were found to translocate to ventral sites and to settle in the vicinity of endogenous neural crest derivatives. However, latex beads coated with fibronectin did not translocate ventrally, but remained associated with cells surrounding the implantation site. Fibronectin is a large glycoprotein with a variety of biological activities and multiple binding domains. Here, the binding activities which might be responsible for immobilization of the fibronectin-coated beads are examined. Latex beads were coated with three types of fragments of the fibronectin molecule representing different functional domains: (i) a 66-kDa fragment containing collagen-binding activity; (ii) a mixture of 45- and 32-kDa fragments containing heparin-binding activity; and (iii) a 120-kDa fragment containing cell-binding activity. The beads coated with fibronectin fragments were injected into the newly formed trunk somites of avian embryos. After injection, beads coated with either the heparin- or the collagen-binding domain translocated ventrally and distributed analogously to uncoated latex beads. In contrast, the majority of beads coated with the fibronectin cell-binding domain did not translocate but remained associated with dermamyotomal cells surrounding the injection site. The cell-binding fragment, however, was not as effective as the intact fibronectin molecule in preventing translocation of the beads. The results suggest that the cell-binding domain is primarily responsible for restriction of fibronectin beads from the ventral neural crest pathway. Because intact fibronectin is more effective at immobilizing beads than is the cell-binding fragment, other binding domains of fibronectin, more efficient coating with intact fibronectin, or crosslinking of intact fibronectin molecules may also play some role in immobilization of the beads at the implantation site.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(85)90015-6DOIArticle
http://www.sciencedirect.com/science/article/pii/0012160685900156PublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1985 Academic Press Inc. Received June 4, 1984; accepted in revised farm October 1, 1984. I thank Dr. Scott Fraser and Dr. Erkki Ruoslahti for critical reading of the manuscript and for helpful suggestions. In addition I gratefully acknowledge the technical assistance of Georgia Guillory and Leena Carriere, and Virginia Bayer for her illustrations. This work was supported by U.S. Public Health Service Grant HD-15527-04 and by Basic Research Grant 1-896 from the March of Dimes Birth Defects Foundation.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)HD-15527-04
March of Dimes Birth Defects Foundation1-896
Issue or Number:1
Record Number:CaltechAUTHORS:20160414-080345720
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160414-080345720
Official Citation:Marianne Bronner-Fraser, Effects of different fragments of the fibronectin molecule on latex bead translocation along neural crest migratory pathways, Developmental Biology, Volume 108, Issue 1, 1985, Pages 131-145, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(85)90015-6. (http://www.sciencedirect.com/science/article/pii/0012160685900156)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66147
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Apr 2016 17:47
Last Modified:03 Oct 2019 09:54

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