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Perturbation of Cranial Neural Crest Migration by the HNK-1 Antibody

Bronner-Fraser, Marianne (1987) Perturbation of Cranial Neural Crest Migration by the HNK-1 Antibody. Developmental Biology, 123 (2). pp. 321-331. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160414-085615839

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Abstract

The HNK-1 antibody recognizes a carbohydrate moiety that is shared by a family of cell adhesion molecules and is also present on the surface of migrating neural crest cells. Here, the effects of the HNK-1 antibody on neural crest cells were examined in vitro and in vivo. When the HNK-1 antibody was added to neural tube explants in tissue culture, neural crest cells detached from laminin substrates but were unaffected on fibronectin substrates. In order to examine the effects of the HNK-1 antibody in vivo, antibody was injected lateral to the mesencephalic neural tube at the onset of cranial neural crest migration. The injected antibody persisted for approximately 16 hr on the injected side of the embryo and appeared to be most prevalent on the surface of neural crest cells. Embryos fixed within the first 24 hr after injection of HNK-1 antibodies (either whole IgMs or small IgM fragments) showed one or more of the following abnormalities: (1) ectopic neural crest cells external to the neural tube, (2) an accumulation of neural crest cells in the lumen of the neural tube, (3) some neural tube anomalies, or (4) a reduction in the neural crest cell volume on the injected side. The ectopic cells and neural tube anomalies persisted in embryos fixed 2 days postinjection. Only embryos having 10 or less somites at the time of injection were affected, suggesting a limited period of sensitivity to the HNK-1 antibody. Control embryos injected with a nonspecific antibody or with a nonblocking antibody against the neural cell adhesion molecule (N-CAM) were unaffected. Previous experiments from this laboratory have demonstrated than an antibody against integrin, a fibronectin and laminin receptor caused defects qualitatively similar to those resulting from HNK-1 antibody injection (M. Bronner-Fraser, J. Cell Biol., 101, 610, 1985). Coinjection of the HNK-1 and integrin antibodies resulted in a greater percentage of affected embryos than with either antibody alone. The additive nature of the effects of the two antibodies suggests that they act at different sites. These results demonstrate that the HNK-1 antibody causes abnormalities in cranial neural crest migration, perhaps by perturbing interactions between neural crest cells and laminin substrates.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(87)90390-3DOIArticle
http://www.sciencedirect.com/science/article/pii/0012160687903903PublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1987 Academic Press, Inc. Received September 12, 1986; accepted in revised form June 1, 1987. I thank Drs. Scott Fraser, Gabrielle Leblanc, James Coulombe, and Roberto Perris for helpful comments on the manuscript, Georgia Guillory for excellent technical assistance, and Thomas Lallier for preparation of small IgM fragments. This work was supported by USPHS Grant HD-15527 and by Basic Research Grant 1-896 from the March of Dimes. M.B.-F. is a Sloan Foundation Fellow.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)HD-15527
March of Dimes1-896
Alfred P. Sloan FoundationUNSPECIFIED
Issue or Number:2
Record Number:CaltechAUTHORS:20160414-085615839
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160414-085615839
Official Citation:Marianne Bronner-Fraser, Perturbation of cranial neural crest migration by the HNK-1 antibody, Developmental Biology, Volume 123, Issue 2, 1987, Pages 321-331, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(87)90390-3. (http://www.sciencedirect.com/science/article/pii/0012160687903903)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66152
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Apr 2016 17:27
Last Modified:03 Oct 2019 09:54

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