CaltechAUTHORS
  A Caltech Library Service

Neural Tube-Derived Factors Influence Differentiation of Neural Crest Cells in Vitro: Effects on Activity of Neurotransmitter Biosynthetic Enzymes

Howard, Marthe J. and Bronner-Fraser, Marianne (1986) Neural Tube-Derived Factors Influence Differentiation of Neural Crest Cells in Vitro: Effects on Activity of Neurotransmitter Biosynthetic Enzymes. Developmental Biology, 117 (1). pp. 45-54. ISSN 0012-1606. http://resolver.caltech.edu/CaltechAUTHORS:20160414-085616155

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20160414-085616155

Abstract

Previously, we have demonstrated that a factor present in chick embryo extract or medium conditioned by neural tube cells supports adrenergic differentiation of some neural crest cells in vitro. These studies have been extended here to examine the effects of this factor(s) on the development of enzymes involved in neurotransmitter biosynthesis. The time course of expression of choline acetyltransferase (ChAT), a marker for cholinergic cells, and dopamine-β-hydroxylase (DBH), a marker for adrenergic cells, was examined in neural crest cell cultures grown under three conditions: (1) in medium containing 10% embryo extract, (2) in medium containing 2% embryo extract, and (3) in medium containing 2% embryo extract that was conditioned by neural tube cells (NTCM). Significant levels of DBH activity were measured in neural crest cell cultures grown in 10% embryo extract containing medium or in NTCM, while only low levels were present in cultures grown in medium containing 2% embryo extract. In contrast, ChAT activity was inhibited by NTCM in comparison to levels in both 10 and 2% embryo extract containing medium. As a preliminary characterization of the factor(s) present in chick embryo extract, we have fractionated embryo extract and find that a pool of 10 kDa or less can support adrenergic differentiation of some neural crest cells. These results suggest that low molecular weight factors present in embryo extract and NTCM support adrenergic expression of neural crest cells, whereas NTCM suppresses cholinergic expression.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(86)90346-5DOIArticle
http://www.sciencedirect.com/science/article/pii/0012160686903465PublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1986 Academic Press, Inc. Received July 16, 1985; accepted in revised form March 20, 1986. We thank Dr. Darwin Berg, Dr. James Coulombe, Dr. Scott Fraser, and Dr. Joseph Margiotta for helpful comments on the manuscript, and Georgia Guillory for technical assistance. This work was partially supported by USPHS Grant 15527-01 and a Basic Research Grant for the March of Dimes Birth Defects Foundation.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)15527-01
March of Dimes Birth Defects FoundationUNSPECIFIED
Record Number:CaltechAUTHORS:20160414-085616155
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160414-085616155
Official Citation:Marthe J. Howard, Marianne Bronner-Fraser, Neural tube-derived factors influence differentiation of neural crest cells in vitro: Effects on activity of neurotransmitter biosynthetic enzymes, Developmental Biology, Volume 117, Issue 1, 1986, Pages 45-54, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(86)90346-5. (http://www.sciencedirect.com/science/article/pii/0012160686903465)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66153
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Apr 2016 17:23
Last Modified:14 Apr 2016 17:23

Repository Staff Only: item control page