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A Lineage-Specific Gene Encoding a Major Matrix Protein of the Sea Urchin Embryo Spicule: II. Structure of the Gene and Derived Sequence of the Protein

Sucov, Henry M. and Benson, Steve and Robinson, John J. and Britten, Roy J. and Wilt, Fred and Davidson, Eric H. (1987) A Lineage-Specific Gene Encoding a Major Matrix Protein of the Sea Urchin Embryo Spicule: II. Structure of the Gene and Derived Sequence of the Protein. Developmental Biology, 120 (2). pp. 507-519. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160414-085616444

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Abstract

A λgt11 cDNA clone isolated by use of a polyclonal antispicule matrix protein antiserum is shown in the accompanying paper [S. C. Benson, H. M. Sucov, L. Stephens, E. H. Davidson, and F. Wilt (1987) Dev. Biol.120, 499–506] to encode a prominent 50-kDa spicule matrix protein (SM50). This clone was used to select homologous genomic recombinants, and the structure of the gene was determined. The SM50 gene occurs once per haploid genome. It contains a single intron located within the 35th codon. A unique transcription initiation site 110 nucleotide pairs prior to the translation start signal was mapped by primer extension. The mRNA is 1895 nucleotides in length, excluding the 3′ poly(A) sequence, and contains a single open reading frame 450 codons in length. Though rare in whole embryo RNA the prevalence of the SM50 mRNA is calculated to be about 1% of the total mRNA in skeletogenic mesenchyme cells. The derived peptide sequence indicates a typical N-terminal signal peptide, and an N-linked glycosylation site near the C terminus. About 45% of the length of the protein is included in a domain composed of consecutive approximate repetitions of a 13-aminoacid element, the consensus sequence of which is Trp-Val-Gly-Asp-Asn-Gln-Ala-^(Leu)_(Trp)-Val-^(Ile)_(Asp)-Asn-^(Gln-Val)_(Pro-Glu). The protein also contains an internal domain unusually rich in proline residues and a very basic C-terminal region.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(87)90254-5DOIArticle
http://www.sciencedirect.com/science/article/pii/0012160687902545PublisherArticle
Additional Information:© 1987 Academic Press, Inc. Received September 4, 1986; accepted in revised form November 6, 1986. This research was supported by NIH Grants HD-05753 (to E.H.D.) and HD-15043 (to F.W.). H.M.S. was supported by NIH Training Grant GM-07616, and J.J.R. was supported by a fellowship from Procter and Gamble.
Funders:
Funding AgencyGrant Number
NIHHD-05753
NIHHD-15043
NIH Predoctoral FellowshipGM-07616
Procter and GambleUNSPECIFIED
Issue or Number:2
Record Number:CaltechAUTHORS:20160414-085616444
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160414-085616444
Official Citation:Henry M. Sucov, Steve Benson, John J. Robinson, Roy J. Britten, Fred Wilt, Eric H. Davidson, A lineage-specific gene encoding a major matrix protein of the sea urchin embryo spicule, Developmental Biology, Volume 120, Issue 2, 1987, Pages 507-519, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(87)90254-5. (http://www.sciencedirect.com/science/article/pii/0012160687902545)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66154
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Apr 2016 16:28
Last Modified:03 Oct 2019 09:54

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