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An Antibody to a Receptor for Fibronectin and Laminin Perturbs Cranial Neural Crest Development in Vivo

Bronner-Fraser, Marianne (1986) An Antibody to a Receptor for Fibronectin and Laminin Perturbs Cranial Neural Crest Development in Vivo. Developmental Biology, 117 (2). pp. 528-536. ISSN 0012-1606. http://resolver.caltech.edu/CaltechAUTHORS:20160414-085616756

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Abstract

Previous studies from this laboratory (M. Bronner-Fraser (1985). J. Cell Biol. 101, 610) have demonstrated that an antibody to a cell surface receptor complex caused alternations in avian neural crest cell migration. Here, these observations are extended to examine the distribution and persistency of injected antibody, the dose dependency of the effect, and the long-term influences of antibody injection. The CSAT antibody, which recognizes a cell surface receptor for fibronectin and laminin, was injected lateral to the mesencephalic neural tube at the onset of cranial neural crest migration. Injected antibody molecules did not cross the midline, but appeared to diffuse throughout the injected half of the mesencephalon, where they remained detectable by immunocytochemistry for about 22 hr. Embryos were examined either during neural crest migration (up to 24 hr after injection) or after formation of neural crest-derived structures (36–48 hr after injection). In those embryo fixed within the first 24 hr, the major defects were a reduction in the neural crest cell number on the injected side, a buildup of neural crest cells within the lumen of the neural tube, and ectopically localized neural crest cells. In embryos allowed to survive for 36 to 48 hr after injection, the neural crest derivatives appeared normal on both the injected and control side, suggesting that the embryos compensated for the reduction in neural crest cell number on the injected side. However, the embryos often had severely deformed neural tubes and ectopic aggregates of neural crest cells. In contrast, several control antibodies had no effect. These findings suggest that the CSAT receptor complex is important in the normal development of the neural crest and neural tube.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(86)90320-9DOIArticle
http://www.sciencedirect.com/science/article/pii/0012160686903209PublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1986 Academic Press, Inc. Received February 19, 1986; accepted in revised form April 28, 1986. I thank Dr. Scott Fraser for helpful comments on the manuscript and Leena Carriere, Carmen Domingo, and Georgia Guillory for their technical assistance. This work was supported by USPHS HD-15527 and by a Basic Research Grant from the March of Dimes Birth Defects Foundation.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)HD-15527
March of Dimes Birth Defects FoundationUNSPECIFIED
Record Number:CaltechAUTHORS:20160414-085616756
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160414-085616756
Official Citation:Marianne Bronner-Fraser, An antibody to a receptor for fibronectin and laminin perturbs cranial neural crest development in vivo, Developmental Biology, Volume 117, Issue 2, 1986, Pages 528-536, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(86)90320-9. (http://www.sciencedirect.com/science/article/pii/0012160686903209)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66155
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Apr 2016 16:18
Last Modified:14 Apr 2016 16:18

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