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Analysis of the Early Stages of Trunk Neural Crest Migration in Avian Embryos Using Monoclonal Antibody HNK-1

Bronner-Fraser, Marianne (1986) Analysis of the Early Stages of Trunk Neural Crest Migration in Avian Embryos Using Monoclonal Antibody HNK-1. Developmental Biology, 115 (1). pp. 44-55. ISSN 0012-1606. http://resolver.caltech.edu/CaltechAUTHORS:20160414-085617023

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Abstract

The monoclonal antibody HNK-1 was used to identify neural crest cells in serial sections of avian embryos to provide a detailed description of the distribution of trunk neural crest cells. The results indicate the presence of three migratory routes in the trunk: (1) a ventral pathway through the anterior sclerotome; (2) a ventral pathway between the neural tube and the posterior sclerotome; and (3) a dorsolateral pathway between the somites and ectoderm. Neural crest cells were first seen entering the anterior half of the sclerotome at about the time the somite begins to dissociate to form the dermomyotome and sclerotome, approximately 5–10 somites rostral to the most recently formed somite. In contrast, neural crest cells were never observed in the posterior sclerotome or in the perinotochordal space. The distribution of neural crest cells was compared with that of injected latex beads which were previously found to translocate along the ventral trunk neural crest pathway (Bronner-Fraser, Dev. Biol. 91, 130, 1982). During the early stages of neural crest migration, injected latex beads were found to extensively colocalize with cells stained by the HNK-1 antibody. Injected latex beads (78%) were found immediately adjacent to HNK-1 positive cells and another 11% were within one cell diameter. The results suggest that latex beads injected into the trunk somites are deposited onto a normal pathway of neural crest migration.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(86)90226-5DOIArticle
http://www.sciencedirect.com/science/article/pii/0012160686902265PublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1986 Academic Press. Inc. Received May 15, 1985; accepted in revised form December 16, 1985. I thank Dr. Scott Fraser and Dr. James Coulombe for their helpful comments on the manuscript, and Georgia Guillory and Jeffrey Pratt for their excellent technical assistance. This work was supported by USPHS Grant HD-15527 and by Basic Research Grant 1-896 from the March of Dimes.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)HD-15527
March of Dimes1-896
Record Number:CaltechAUTHORS:20160414-085617023
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160414-085617023
Official Citation:Marianne Bronner-Fraser, Analysis of the early stages of trunk neural crest migration in avian embryos using monoclonal antibody HNK-1, Developmental Biology, Volume 115, Issue 1, 1986, Pages 44-55, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(86)90226-5. (http://www.sciencedirect.com/science/article/pii/0012160686902265)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66156
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Apr 2016 17:31
Last Modified:14 Apr 2016 17:31

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