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Expression of Myosin Heavy Chain Gene in the Sea Urchin: Coregulation with Muscle Actin Transcription in Early Development

Rose, Samuel J. and Rosenberg, Marvin J. and Britten, Roy J. and Davidson, Eric H. (1987) Expression of Myosin Heavy Chain Gene in the Sea Urchin: Coregulation with Muscle Actin Transcription in Early Development. Developmental Biology, 123 (1). pp. 115-124. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160414-093724349

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Abstract

A fragment of a Strongylocentrotus purpuratus (Sp) myosin heavy chain (MHC) gene was isolated from a genomic recombinant DNA library by cross-reaction with a cloned Drosophila melanogaster (Dm) MHC probe. A portion of a 227-nucleotide Sp coding sequence that is included in this fragment predicts a peptide very closely homologous with a region of the Dm sequence. The MHC gene sequence is present in a single copy per haploid Sp genome, and the gene is utilized in adult as well as embryonic muscle. The quantity of MHC transcript was measured in embryos of various stages by single-strand RNA probe excess titration. Transcripts are not observed until postgastrular stages, after which they accumulate rapidly. The time course of accumulation closely parallels that measured earlier for muscle actin message.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(87)90433-7DOIArticle
http://www.sciencedirect.com/science/article/pii/0012160687904337PublisherArticle
Additional Information:© 1987 Academic Press, Inc. Received January 2, 1987; accepted in revised form April 21, 1987. We wish to thank Ms. Diane Chen (a Caltech Summer Undergraduate Research Fellow) and Ms. Cathy Shapiro for excellent technical assistance. The generous sharing of clones and unpublished data from Drs. S. Bernstein and C. Rozek is gratefully acknowledged. Mr. Kurt Eakle devised the computer program, based on the Chou and Fasmin (1978) rules, which was used to analyze our MHC exon. This research was supported by an NIH grant (GM-20927). S.J.R. was supported in part by a postdoctoral fellowship from the Muscular Dystrophy Association.
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Funding AgencyGrant Number
NIHGM-20927
Muscular Dystrophy AssociationUNSPECIFIED
Issue or Number:1
Record Number:CaltechAUTHORS:20160414-093724349
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160414-093724349
Official Citation:Samuel J. Rose, Marvin J. Rosenberg, Roy J. Britten, Eric H. Davidson, Expression of myosin heavy chain gene in the sea urchin: Coregulation with muscle actin transcription in early development, Developmental Biology, Volume 123, Issue 1, 1987, Pages 115-124, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(87)90433-7. (http://www.sciencedirect.com/science/article/pii/0012160687904337)
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ID Code:66159
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Apr 2016 17:20
Last Modified:03 Oct 2019 09:54

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