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Effects of lsotretinoin on the Behavior of Neural Crest Cells in Vitro

Smith-Thomas, Linda and Lott, Ira and Bronner-Fraser, Marianne (1987) Effects of lsotretinoin on the Behavior of Neural Crest Cells in Vitro. Developmental Biology, 123 (1). pp. 276-281. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160414-093724625

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Abstract

Isotretinoin (13-cis-retinoic acid), an anti-acne medication, has been found to cause severe birth defects which affect the craniofacial elements, ear, heart, thymus, and central nervous system. Many of these structures receive contributions from the cranial neural crest. Here, we examine the possibility that these teratogenic effects are due to disturbances in neural crest development. Cranial and trunk neural crest explant cultures were exposed to different concentrations of isotretinoin and the cell morphology was monitored at daily intervals. Treated neural crest cells often became rounded or spindle shaped, separated from their neighbors, and frequently detached from the substrate or clumped together. In contrast, neural tube cells and cardiac fibroblasts were relatively unaffected by the drug. These results suggest that isotretinoin selectively affects neural crest cells by decreasing their cell-substratum adhesion.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(87)90449-0DOIArticle
http://www.sciencedirect.com/science/article/pii/0012160687904490PublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1987 Academic Press, Inc. Received November 3, 1986; accepted in revised farm April 20, 1987. We are grateful to Dr. Peter Sorter and Hoffman La-Roche for providing us with isotretinoin. We thank Drs. Gabrielle LeBlanc, Danuta Krotoski, James Coulombe, Georgia Guillory, and Tom Lallier for their helpful comments on the manuscript. This work was supported by Monsanto Company and Basic Research Grant 1-896 from the March of Dimes Birth Defects Foundation. M. Bronner-Fraser is a Sloan Fellow.
Funders:
Funding AgencyGrant Number
Monsanto CompanyUNSPECIFIED
March of Dimes Birth Defects Foundation1-896
Alfred P. Sloan FoundationUNSPECIFIED
Issue or Number:1
Record Number:CaltechAUTHORS:20160414-093724625
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160414-093724625
Official Citation:Linda Smith-Thomas, Ira Lott, Marianne Bronner-Fraser, Effects of isotretinoin on the behavior of neural crest cells in vitro, Developmental Biology, Volume 123, Issue 1, 1987, Pages 276-281, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(87)90449-0. (http://www.sciencedirect.com/science/article/pii/0012160687904490)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66160
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Apr 2016 21:13
Last Modified:03 Oct 2019 09:54

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