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Alterations in the Xenopus Retinotectal Projection by Antibodies to Xenopus N-CAM

Fraser, Scott E. and Carhart, M. Scott and Murray , Ben A. and Chuong, Cheng-Ming and Edelman, Gerald M. (1988) Alterations in the Xenopus Retinotectal Projection by Antibodies to Xenopus N-CAM. Developmental Biology, 129 (1). pp. 217-230. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160414-093725132

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Abstract

The patterned neural projection from the eye to the optic tectum of lower vertebrates (the retinotectal projection) has been proposed to be ordered by interactions between the optic nerve fibers and their surrounding tissues. To investigate the role of one such defined cell interaction, agarose implants containing antibodies to the neural cell adhesion molecule, N-CAM, were inserted into the tectum of the African clawed frog, Xenopus laevis. Both monoclonal and polyclonal antibodies against N-CAM reversibly and specifically distorted the pattern of the retinotectal projection, decreasing the precision of the projection as determined by electrophysiological techniques as well decreasing the density of retinal innervation of the tectum and the branching of single axons as determined by horseradish peroxidase tracing. The anatomical effects became maximal at 4 to 6 days after implantation and returned to undetectable levels by 2 weeks, whereas the physiological effects became maximal by 8 to 10 days and a normal physiological map was reestablished within 4 weeks. The results are consistent with the hypothesis that anti-N-CAM antibodies perturb the ongoing growth and retraction of the terminal arbors of the optic nerve fibers, such that a region of the tectum becomes largely denuded of fibers. The physiological defects may then be a consequence both of the initial retraction of optic nerve terminals and of the rapid ingrowth of the perturbed and neighboring optic nerve fibers into the denuded region after the antibodies were cleared from the tectum. These results support the concept of a major role for N-CAM-mediated adhesion during map regeneration and maintenance.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(88)90176-5DOIArticle
http://www.sciencedirect.com/science/article/pii/0012160688901765PublisherArticle
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 1988 Academic Press, Inc. Accepted May 20, 1988. This work was supported by grants from the NIH (DK-04256 and HD-09635), a Senator Jacob Javits Center of Excellence grant (NS-22789), a grant from the NSF (BNS-8608356), and a McKnight Scholar Award.
Funders:
Funding AgencyGrant Number
NIHDK-04256
NIHHD-09635
NIHNS-22789
NSFBNS-8608356
McKnight FoundationUNSPECIFIED
Issue or Number:1
Record Number:CaltechAUTHORS:20160414-093725132
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160414-093725132
Official Citation:Scott E. Fraser, M.Scott Carhart, Ben A. Murray, Cheng-Ming Chuong, Gerald M. Edelman, Alterations in the Xenopus retinotectal projection by antibodies to Xenopus N-CAM, Developmental Biology, Volume 129, Issue 1, 1988, Pages 217-230, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(88)90176-5. (http://www.sciencedirect.com/science/article/pii/0012160688901765)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66162
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Apr 2016 17:18
Last Modified:03 Oct 2019 09:54

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